About Gene List

PF3D7_0717600 (IMC32)

Genome location: Pf3D7_07_v3:758,490..763,360(-)

Genome classification: Core

Function and Localization

Product Description: inner membrane complex protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0070258 inner membrane pellicle complex
Component GO:0005634 nucleus

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite not expressed N/A N/A
Ring not expressed 0.11 0.94
Trophozoite not expressed 0.78 0.60
Schizont possibly expressed 1.46 0.38
Gametocyte expressed 1.68 0.36

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0717600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.329 | MFS: -2.592 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0615300)

  • Relative Growth Rate: 0.04 ± 0.09
  • Confidence: 6.27

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A143ZVJ7

"Best" AlphaFill ligand hit: FAR (farnesyl, Local RMSD=1.06) with 6K1Z (Global RMSD=25.74)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_119267

Most Similar Human Ortholog: H0YBP4

TM-align score: 0.81 | RMSD: 1.50

Seq Identity: 0.07 | Length: 43 / 1053

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000136436 calcium binding and coiled-coil domain 2

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 4 38 22 49
disruptive 14 50 41 115
missense 11 49 40 94

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 12 74 38 57
disruptive 33 181 99 259
missense 19 147 83 162

PlasmoDB Total SNPs: 237

Non-coding: 188 | Synonymous: 32 | Nonsynonymous: 17 | Stop Codon: 0

Protein Information

Protein Length: 1053 | Molecular Weight (kDa): 125.439

UniProt ID(s): A0A143ZVJ7

PDB ID(s): None

Isoelectric Point: 8.1

Protein Domain Annotations:

Source Family ID Description
InterPro N/A N/A
PFam N/A N/A
Superfamily N/A N/A

Associated Publications

PMID Title Authors DOI/Link
23181666 Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. Oehring SC, Woodcroft BJ, ..., Voss TS 10.1186/gb-2012-13-11-r108
33593973 Identification and Molecular Dissection of I MC32, a Conserved Toxoplasma Inner Membrane Complex Protein That Is Essential for Parasite Replication Torres JA, Pasquarelli RR, ..., Bradley PJ 10.1128/mBio.03622-20
37782662 Identification of IMC43, a novel IMC protein that collaborates with IMC32 to forman essential daughter bud assembly complex in Toxoplasma gondii Pasquarelli RR, Back PS, ..., Bradley PJ 10.1371/journal.ppat.1011707