PF3D7_0717700

Genome location: Pf3D7_07_v3:764,201..766,451(-)

Genome classification: Core

Function and Localization

Product Description: serine--tRNA ligase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 6.1.1.11 (Serine--tRNA ligase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005829	cytosol
Function	GO:0005524	ATP binding
Function	GO:0004828	serine-tRNA ligase activity
Function	GO:0000049	tRNA binding
Process	GO:0006434	seryl-tRNA aminoacylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.66	0.71
Trophozoite	expressed	1.08	0.46
Schizont	expressed	0.43	0.73
Gametocyte	expressed	0.85	0.55

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0717700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.127 | MFS: -3.11 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1431700)

Relative Growth Rate: 0.25 ± 0.66
Confidence: 2.21

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0615400)

Modification: Disrupted | RMgm-2127

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IBS3

"Best" AlphaFill ligand hit: SSA (5'-o-(n-(l-seryl)-sulfamoyl)adenosine, Local RMSD=0.26) with 6OTE (Global RMSD=8.09)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
6.1.1.11	serine-tRNA ligase	GSSGsiRNAglycinealanine3'-dATPcysteinethreonineSB-217452serinamidediphosphatetRNASer-C-CPoly-arginine...

Orthology to BindingDB Entries:

UniProt	Protein Name	Homology Source	BindingDB Ligands
P49591	Serine--tRNA ligase, cytoplasmic	OMA, OrthoDB	SerSASerSA
P95689	Serine--tRNA ligase	OMA	CHEMBL4452350SerSACHEMBL4446951CHEMBL4561900...
P0A8L1	Serine--tRNA ligase	OMA	CHEMBL4208640SerSASerSACHEMBL4452350...

Orthology Information

Ortholog Group (OrthoMCL): OG6_100275

Most Similar Human Ortholog: M0R259

TM-align score: 0.87 | RMSD: 1.76

Seq Identity: 0.29 | Length: 300 / 539

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000031698	seryl-tRNA synthetase 1
ENSG00000104835	seryl-tRNA synthetase 2, mitochondrial
ENSG00000269547	novel protein
ENSG00000282835	novel protein
ENSG00000283104	seryl-tRNA synthetase 2, mitochondrial

Protein Information

Protein Length: 539 | Molecular Weight (kDa): 62.454

UniProt ID(s): A0A5J6C6U5, A0A5J6C835, A0A5J6C838, A0A5J6C857, A0A5J6C8C0, A0A5J6C8E2, A0A5J6C8X3, A0A5J6C8Z5, A0A5J6C9G5, A0A5J6C9H7, A0A5J6CA01, A0A5J6CAA9, A0A5J6CAQ8, A0A5J6CAW2, Q8IBS3

PDB ID(s): None

Isoelectric Point: 6.93

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002314	Aminoacyl-tRNA synthetase, class II (G/ P/ S/T)
InterPro	IPR002317	Serine-tRNA ligase, type1
InterPro	IPR010978	Class I and II aminoacyl-tRNA synthetase, tRNA-binding arm
InterPro	IPR015866	Serine-tRNA synthetase, type1, N-terminal
InterPro	IPR033729	Serine-tRNA ligase catalytic core domain
PFam	PF00587	Aminoacyl-tRNA synthetase, class II (G/ P/ S/T)
PFam	PF02403	Serine-tRNA synthetase, type1, N-terminal
Superfamily	SSF46589	Class I and II aminoacyl-tRNA synthetase, tRNA-binding arm
Superfamily	SSF55681

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	17	15	29
disruptive	5	31	18	54
missense	5	31	18	42

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	4	44	16	34
disruptive	14	72	40	101
missense	12	62	32	66

PlasmoDB Total SNPs: 69

Non-coding: 53 | Synonymous: 11 | Nonsynonymous: 5 | Stop Codon: 0