PF3D7_0718100 (EST)

Genome location: Pf3D7_07_v3:795,957..805,184(-)

Genome classification: Core

Function and Localization

Product Description: exported serine/threonine protein kinase

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020036	Maurer's cleft
Component	GO:1903561	extracellular vesicle
Component	GO:0016020	membrane
Function	GO:0004674	protein serine/threonine kinase activity
Process	GO:0006468	protein phosphorylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.08	0.96
Trophozoite	expressed	0.68	0.64
Schizont	expressed	0.21	0.88
Gametocyte	expressed	0.90	0.57

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0718100

Resistome Missense Mutations: F2330C

Resistome Compounds with Missense Mutations: 3-Hydroxypropanamidine 22

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.152 | #Insertions: 26

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0615800)

Relative Growth Rate: 0.12 ± 0.35
Confidence: 3.51

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IBR9

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.11.1	non-specific serine/threonine protein kinase	ATPKClDTTP53DRBEDTATBCAKN-93K252aCKI-7LAALSTAACS...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532974

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	14	86	42	125
disruptive	56	187	125	298
missense	40	168	106	258

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	24	144	75	140
disruptive	104	397	197	447
missense	68	333	171	346

PlasmoDB Total SNPs: 213

Non-coding: 67 | Synonymous: 86 | Nonsynonymous: 59 | Stop Codon: 1

Protein Information

Protein Length: 2695 | Molecular Weight (kDa): 317.692

UniProt ID(s): Q8IBR9, Q94658, Q9GVB3

PDB ID(s): None

Isoelectric Point: 8.13

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000719	Protein kinase domain
InterPro	IPR011009	Protein kinase-like domain superfamily
PFam	N/A	N/A
Superfamily	SSF56112	Protein kinase-like domain superfamily

PMID	Title	Authors	DOI/Link
9108547	A putative Plasmodium falciparum exported serine/threonine protein kinase.	Kun JF, Hibbs AR, ..., Anders RF	10.1016/s0166-6851(96)02805-8
18614010	Exported proteins required for virulence and rigidity of Plasmodiumfalciparum-infected human erythrocytes.	Maier AG, Rug M, ..., Cowman AF	10.1016/j.cell.2008.04.051
33608523	20S proteasomes secreted by the malaria parasite promote its growth.	Dekel E, Yaffe D, ..., Regev-Rudzki N	10.1038/s41467-021-21344-8