Product Description: NIMA related kinase 4
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Function | GO:0005524 | ATP binding |
| Function | GO:0004674 | protein serine/threonine kinase activity |
| Process | GO:0006468 | protein phosphorylation |
| Process | GO:0007165 | signal transduction |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.00 | 1.00 |
| Trophozoite | not expressed | 0.01 | 0.99 |
| Schizont | not expressed | 0.01 | 0.99 |
| Gametocyte | expressed | 0.63 | 0.70 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0719200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.195 | MFS: -2.384 | #Insertions: 0
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0616700)
RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PY17X_0619400)
Modification: Disrupted | RMgm-4817
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C0H4N8
"Best" AlphaFill ligand hit: OCT (n-octane, Local RMSD=0.08) with 1APM (Global RMSD=4.39)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.11.1 | non-specific serine/threonine protein kinase | ATPKClDTTP53DRBEDTATBCAKN-93K252aCKI-7LAALSTAACS... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P46734 | Dual specificity mitogen-activated protein kinase kinase 3 | Homo sapiens | HOGENOM | US8546404, 6CHEMBL3746157CHEMBL3746484CHEMBL3747095... |
| P45985 | Dual specificity mitogen-activated protein kinase kinase 4 | Homo sapiens | HOGENOM | US11370770, Compound 6aUS11370770, Compound 6bUS11370770, Compound 6cUS11370770, Compound 6d... |
| Q13188 | Serine/threonine-protein kinase 3 | Homo sapiens | HOGENOM | BI-D1870US10710978, Compound I-1US10710978, Compound I-9US10710978, Compound I-12... |
| Q8TDX7 | Serine/threonine-protein kinase Nek7 | Homo sapiens | HOGENOM, OrthoDB | US11713321, Example 1US11713321, Example 6US11713321, Example 7US11713321, Example 8... |
| P31938 | Dual specificity mitogen-activated protein kinase kinase 1 | Mus musculus | HOGENOM | CHEMBL188343CHEMBL1998165US8575391, Q |
| Q01986 | Dual specificity mitogen-activated protein kinase kinase 1/2 | HOGENOM | CHEMBL3326331 | |
| P51955 | Serine/threonine-protein kinase Nek2 | HOGENOM, OrthoDB | US9505766, 10US9505766, 11US9505766, 12US9505766, 13... | |
| O14733 | Dual specificity mitogen-activated protein kinase kinase 7 | HOGENOM | CHEBI:67559CHEMBL507249CHEMBL4095253CHEMBL1801950... | |
| Q9P289 | Serine/threonine-protein kinase 26 | Homo sapiens | HOGENOM | US9156845, 4US9156845, 6CHEMBL3393445CHEMBL3393450... |
| P52564 | Dual specificity mitogen-activated protein kinase kinase 6 | Homo sapiens | HOGENOM | Staurosporine, 8L-783277Staurosporine, 8CHEMBL4568087... |
| P36506 | Dual specificity mitogen-activated protein kinase kinase 2 | HOGENOM | PD-98059CHEMBL3326332CHEMBL3326319CHEMBL3326325... | |
| Q86SG6 | Serine/threonine-protein kinase Nek8 | OrthoDB | Staurosporine, 8Staurosporine, 8 | |
| Q6P3R8 | Serine/threonine-protein kinase Nek5 | Homo sapiens | OrthoDB | CHEMBL3745885Staurosporine, 8Staurosporine, 8 |
| Q8TD19 | Serine/threonine-protein kinase Nek9 | Homo sapiens | OrthoDB | Staurosporine, 8Staurosporine, 8CHEMBL4793380 |
| Q9HC98 | Serine/threonine-protein kinase Nek6 | Homo sapiens | OrthoDB | CHEMBL1272140CHEMBL1271644CHEMBL1271870med.21724, Compound 4... |
| Q8NG66 | Serine/threonine-protein kinase Nek11 | Homo sapiens | OrthoDB | Staurosporine, 8Staurosporine, 8 |
| P51957 | Serine/threonine-protein kinase Nek4 | Homo sapiens | OrthoDB | CHEMBL2171124Staurosporine, 8CHEMBL4550702CHEMBL4568087... |
| Q96PY6 | Serine/threonine-protein kinase Nek1 | OrthoDB | US9051313, StaurosporineUS9051313, StaurosporineCHEMBL3745885CHEMBL1276848... |
Ortholog Group (OrthoMCL): OG6_105432
Most Similar Human Ortholog: P51957
TM-align score: 0.87 | RMSD: 2.83
Seq Identity: 0.38 | Length: 299 / 310
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000114904 | NIMA related kinase 4 |
Protein Length: 310 | Molecular Weight (kDa): 36.35
UniProt ID(s): C0H4N8
PDB ID(s): None
Isoelectric Point: 9.69
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000719 | Protein kinase domain |
| InterPro | IPR008271 | Serine/threonine-protein kinase, active site |
| InterPro | IPR011009 | Protein kinase-like domain superfamily |
| InterPro | IPR017441 | Protein kinase, ATP binding site |
| PFam | PF00069 | Protein kinase domain |
| Superfamily | SSF56112 | Protein kinase-like domain superfamily |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 8 | 6 | 21 |
| disruptive | 0 | 0 | 1 | 8 |
| missense | 0 | 0 | 1 | 6 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 16 | 13 | 22 |
| disruptive | 0 | 9 | 5 | 36 |
| missense | 0 | 5 | 4 | 21 |
PlasmoDB Total SNPs: 161
Non-coding: 145 | Synonymous: 12 | Nonsynonymous: 2 | Stop Codon: 2
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 15970588 | A NIMA-related protein kinase is essential for completion of the sexual cycle ofmalaria parasites. | Reininger L, Billker O, ..., Doerig C | 10.1074/jbc.M504523200 |
| 36810637 | A non-canonical sensing pathway mediates Plasmodium adaptation to amino aciddeficiency | Marreiros IM, Marques S, ..., Mota MM | 10.1038/s42003-023-04566-y |