PF3D7_0721300 (DDX31)

Genome location: Pf3D7_07_v3:921,511..925,761(+)

Genome classification: Core

Function and Localization

Product Description: ATP-dependent DNA helicase DDX31

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.4.12 (DNA helicase);3.6.4.13 (RNA helicase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005730	nucleolus
Component	GO:0005634	nucleus
Function	GO:0005524	ATP binding
Function	GO:0008094	ATP-dependent activity, acting on DNA
Function	GO:0008186	ATP-dependent activity, acting on RNA
Function	GO:0003678	DNA helicase activity
Function	GO:0003723	RNA binding
Function	GO:0003724	RNA helicase activity
Process	GO:0016070	RNA metabolic process
Process	GO:0042254	ribosome biogenesis

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.73	0.66
Trophozoite	expressed	0.58	0.69
Schizont	expressed	0.13	0.92
Gametocyte	expressed	0.66	0.64

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0721300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.149 | MFS: -2.847 | #Insertions: 0

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0618800)

Relative Growth Rate: 0.59 ± 0.14
Confidence: 5.30

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0618800)

Modification: Disrupted | RMgm-2143

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IBN8

"Best" AlphaFill ligand hit: KEG (12-tungstophosphate, Local RMSD=0.05) with 5SUQ (Global RMSD=2.13)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.4.12	DNA helicase	No BRENDA inhibitors
3.6.4.13	RNA helicase	ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102676

Most Similar Human Ortholog: Q9H8H2

TM-align score: 0.59 | RMSD: 4.22

Seq Identity: 0.30 | Length: 547 / 899

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000125485	DEAD-box helicase 31

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	42	21	51
disruptive	69	162	65	169
missense	40	88	44	112

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	7	78	39	69
disruptive	117	313	135	298
missense	59	184	90	160

PlasmoDB Total SNPs: 323

Non-coding: 179 | Synonymous: 89 | Nonsynonymous: 54 | Stop Codon: 1

Protein Information

Protein Length: 899 | Molecular Weight (kDa): 105.03

UniProt ID(s): A0A346QRV1, A0A5J6CZ29, A7U5X0, Q8IBN8

PDB ID(s): None

Isoelectric Point: 9.09

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000629	ATP-dependent RNA helicase DEAD-box, conserved site
InterPro	IPR001650	Helicase, C-terminal
InterPro	IPR011545	DEAD/DEAH box helicase domain
InterPro	IPR014001	Helicase superfamily 1/2, ATP-binding domain
InterPro	IPR025313	Domain of unknown function DUF4217
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF00270	DEAD/DEAH box helicase domain
PFam	PF00271	Helicase, C-terminal
PFam	PF13959	Domain of unknown function DUF4217
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase