Product Description: protein pelota homolog, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.1.-.- (Acting on ester bonds.)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Process | GO:0048255 | mRNA stabilization |
| Process | GO:0070651 | nonfunctional rRNA decay |
| Process | GO:0000280 | nuclear division |
| Process | GO:0070966 | nuclear-transcribed mRNA catabolic process, no-go decay |
| Process | GO:0032790 | ribosome disassembly |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.17 | 0.91 |
| Trophozoite | expressed | 0.25 | 0.84 |
| Schizont | expressed | 0.08 | 0.94 |
| Gametocyte | not expressed | 0.13 | 0.92 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0722100
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -0.646 | #Insertions: 4
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0619600)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0619600)
Modification: Disrupted | RMgm-2146
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I6Z3
"Best" AlphaFill ligand hit: AGS (phosphothiophosphoric acid-adenylate ester, Local RMSD=1.78) with 6T4H (Global RMSD=13.20)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.-.- | Ex: 5-phospho-D-xylono-1,4-lactonase | paraoxonatropineethylene terephthalatediisopropyl fluorophosphonatebis(2-hydroxyethyl) terephthalic acidphosphoric acid-bis-4-nitrophenyl ester |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101450
Most Similar Human Ortholog: Q9BRX2
TM-align score: 0.93 | RMSD: 1.97
Seq Identity: 0.36 | Length: 377 / 403
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000152684 | pelota mRNA surveillance and ribosome rescue factor |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 11 | 5 | 23 |
| disruptive | 5 | 23 | 23 | 52 |
| missense | 5 | 22 | 22 | 49 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 28 | 17 | 29 |
| disruptive | 8 | 66 | 53 | 113 |
| missense | 7 | 53 | 44 | 75 |
PlasmoDB Total SNPs: 121
Non-coding: 97 | Synonymous: 14 | Nonsynonymous: 10 | Stop Codon: 0
Protein Length: 403 | Molecular Weight (kDa): 47.006
UniProt ID(s): Q8I6Z3
PDB ID(s): None
Isoelectric Point: 8.96
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004405 | Translation release factor pelota |
| InterPro | IPR005140 | eRF1 domain 1/Pelota-like |
| InterPro | IPR005141 | eRF1 domain 2 |
| InterPro | IPR005142 | eRF1 domain 3 |
| InterPro | IPR029064 | 50S ribosomal protein L30e-like |
| InterPro | IPR038069 | Pelota/DOM34, N-terminal domain |
| PFam | PF03463 | eRF1 domain 1/Pelota-like |
| PFam | PF03464 | eRF1 domain 2 |
| PFam | PF03465 | eRF1 domain 3 |
| Superfamily | SSF159065 | Pelota/DOM34, N-terminal domain |
| Superfamily | SSF53137 | 50S ribosomal protein L30e-like |
| Superfamily | SSF55315 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 32469313 | Plasmodium falciparum translational machinery condones polyadenosine repeats | Pavlovic Djuranovic S, Erath J, ..., Djuranovic S | 10.7554/eLife.57799 |