PF3D7_0722200 (RALP1)

Genome location: Pf3D7_07_v3:946,077..949,317(+)

Genome classification: Core

Function and Localization

Product Description: rhoptry-associated leucine zipper-like protein 1

SignalP Peptide: MNFFFFFIIVYCFISSFYLIKS

# Transmembrane Domains: 1

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:1990225	rhoptry neck
Function	GO:0046789	host cell surface receptor binding
Process	GO:0044409	entry into host

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.18	0.92
Trophozoite	possibly expressed	0.16	0.91
Schizont	expressed	3.24	0.15
Gametocyte	possibly expressed	0.19	0.89

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0722200

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.241 | MFS: -2.644 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0619700)

Relative Growth Rate: 0.19 ± 0.34
Confidence: 3.52

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0619700)

Modification: Tagged | RMgm-4717

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IBN1

"Best" AlphaFill ligand hit: GDP (guanosine-5'-diphosphate, Local RMSD=0.23) with 8FWP (Global RMSD=24.17)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532308

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	22	15	40
disruptive	28	78	53	114
missense	14	57	48	84

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	7	51	30	45
disruptive	46	186	97	191
missense	22	131	78	118

PlasmoDB Total SNPs: 175

Non-coding: 72 | Synonymous: 66 | Nonsynonymous: 37 | Stop Codon: 0

Protein Information

Protein Length: 749 | Molecular Weight (kDa): 87.879

UniProt ID(s): A0A193PDD1, A0A193PDF2, A0A193PDH1, A0A193PDI0, A0A193PDY0, A0A193PDZ4, A0A193PDZ9, A0A193PES0, A0A193PEU1, A0A193PEX6, Q8IBN1

PDB ID(s): None

Isoelectric Point: 5.14

Protein Domain Annotations:

Source	Family ID	Description
InterPro	N/A	N/A
PFam	N/A	N/A
Superfamily	N/A	N/A

Associated Publications

PMID	Title	Authors	DOI/Link
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
18174339	Characterization of a conserved rhoptry-associated leucine zipper-like protein inthe malaria parasite Plasmodium falciparum.	Haase S, Cabrera A, ..., Gilberger TW	10.1128/IAI.00144-07
24002067	RALP1 is a rhoptry neck erythrocyte-binding protein of Plasmodium falciparummerozoites and a potential blood-stage vaccine candidate antigen.	Ito D, Hasegawa T, ..., Tsuboi T	10.1128/IAI.00690-13
27692771	Identification of Plasmodium falciparum reticulocyte binding protein homologue5-interacting protein, PfRipr, as a highly conserved blood-stage malaria vaccinecandidate.	Ntege EH, Arisue N, ..., Tsuboi T	10.1016/j.vaccine.2016.09.028
31552198	Expression and Localization Profiles of Rhoptry Proteins in Plasmodium bergheiSporozoites	Tokunaga N, Nozaki M, ..., Ishino T	10.3389/fcimb.2019.00316
23201113	Systematic analysis of natural antibody responses to P. falciparum merozoiteantigens by protein arrays	Fan YT, Wang Y, ..., Chen JH	10.1016/j.jprot.2012.11.020
31964745	Peripheral Merozoite Surface Proteins Are Targets of Naturally Acquired Immunityagainst Malaria in both India and Ghana	Garcia-Senosiain A, Kana IH, ..., Theisen M	10.1128/IAI.00778-19
33886601	Suitability of IgG responses to multiple Plasmodium falciparum antigens asmarkers of transmission intensity and pattern	Kyei-Baafour E, Oppong M, ..., Ofori MF	10.1371/journal.pone.0249936