PF3D7_0723000

Genome location: Pf3D7_07_v3:968,088..970,023(-)

Genome classification: Core

Function and Localization

Product Description: tRNAHis guanylyltransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.7.7.79 (tRNA(His) guanylyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0008193	tRNA guanylyltransferase activity
Process	GO:0008033	tRNA processing

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.15	0.92
Trophozoite	expressed	0.11	0.93
Schizont	not expressed	0.03	0.98
Gametocyte	not expressed	0.13	0.92

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0723000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.14 | MFS: -3.009 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0620500)

Relative Growth Rate: 0.05 ± 0.05
Confidence: 7.41

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IBM3

"Best" AlphaFill ligand hit: ATP (adenosine-5'-triphosphate, Local RMSD=0.03) with 3WBZ (Global RMSD=3.48)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.7.7.79	tRNAHis guanylyltransferase	ADPAMP5,5'-dithiobis(2-nitrobenzoic)acid

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_103009

Most Similar Human Ortholog: Q9NWX6

TM-align score: 0.82 | RMSD: 2.33

Seq Identity: 0.40 | Length: 260 / 299

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000113272	tRNA-histidine guanylyltransferase 1 like

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	11	5	14
disruptive	8	15	9	28
missense	3	14	9	24

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	2	18	16	21
disruptive	15	33	20	42
missense	5	27	15	31

PlasmoDB Total SNPs: 122

Non-coding: 109 | Synonymous: 2 | Nonsynonymous: 11 | Stop Codon: 0

Protein Information

Protein Length: 299 | Molecular Weight (kDa): 36.192

UniProt ID(s): Q8IBM3

PDB ID(s): None

Isoelectric Point: 8.43

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR007537	tRNAHis guanylyltransferase Thg1
InterPro	IPR024956	tRNAHis guanylyltransferase catalytic domain
InterPro	IPR025845	Thg1 C-terminal domain
PFam	PF04446	tRNAHis guanylyltransferase catalytic domain
PFam	PF14413	Thg1 C-terminal domain
Superfamily	N/A	N/A