Product Description: metallo-hydrolase/oxidoreductase, putative
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: 3.1.26.11 (Ribonuclease Z)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005634 | nucleus |
| Function | GO:0042781 | 3'-tRNA processing endoribonuclease activity |
| Process | GO:0034414 | tRNA 3'-trailer cleavage, endonucleolytic |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.17 | 0.92 |
| Trophozoite | expressed | 0.56 | 0.68 |
| Schizont | expressed | 0.11 | 0.93 |
| Gametocyte | expressed | 0.44 | 0.75 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0723700
Resistome Missense Mutations: D957Y
Resistome Compounds with Missense Mutations: BI-2536
Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.48 | MFS: -2.518 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1328700)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IBL6
"Best" AlphaFill ligand hit: PGO (s-1,2-propanediol, Local RMSD=0.35) with 2E7Y (Global RMSD=6.19)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.1.26.11 | tRNase Z | BRENDA SOAP query unsuccessful |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100263
Most Similar Human Ortholog: Q9H777
TM-align score: 0.80 | RMSD: 3.26
Seq Identity: 0.31 | Length: 332 / 1032
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000006744 | elaC ribonuclease Z 2 |
| ENSG00000141642 | elaC ribonuclease Z 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 15 | 44 | 29 | 61 |
| disruptive | 64 | 115 | 66 | 150 |
| missense | 30 | 74 | 51 | 112 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 23 | 96 | 41 | 58 |
| disruptive | 100 | 233 | 104 | 263 |
| missense | 41 | 163 | 76 | 178 |
PlasmoDB Total SNPs: 184
Non-coding: 32 | Synonymous: 83 | Nonsynonymous: 69 | Stop Codon: 0
Protein Length: 1032 | Molecular Weight (kDa): 119.659
UniProt ID(s): Q8IBL6
PDB ID(s): None
Isoelectric Point: 7.32
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001279 | Metallo-beta-lactamase |
| InterPro | IPR036866 | Ribonuclease Z/Hydroxyacylglutathione hydrolase-like |
| PFam | PF12706 | Metallo-beta-lactamase |
| Superfamily | SSF56281 | Ribonuclease Z/Hydroxyacylglutathione hydrolase-like |
| Superfamily | SSF69349 |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |