PF3D7_0724300 (COQ3)

Genome location: Pf3D7_07_v3:1,021,112..1,022,659(+)

Genome classification: Core

Function and Localization

Product Description: ubiquinone biosynthesis O-methyltransferase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.1.1.222 (2-polyprenyl-6-hydroxyphenol methylase);2.1.1.64 (3-demethylubiquinol 3-O-methyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005739	mitochondrion
Function	GO:0008689	3-demethylubiquinone-9 3-O-methyltransferase activity
Process	GO:0006744	ubiquinone biosynthetic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.05	0.97
Trophozoite	possibly expressed	0.29	0.81
Schizont	not expressed	0.07	0.95
Gametocyte	not expressed	0.28	0.83

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0724300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.79 | MFS: -2.616 | #Insertions: 0

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0621800)

Relative Growth Rate: 0.31 ± 0.12
Confidence: 5.60

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0621800)

Modification: Disrupted | RMgm-2153

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IBK9

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.1.1.222	2-polyprenyl-6-hydroxyphenol methylase	No BRENDA inhibitors
2.1.1.64	3-demethylubiquinol 3-O-methyltransferase	No BRENDA inhibitors

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102172

Most Similar Human Ortholog: F6WGP0

TM-align score: 0.68 | RMSD: 2.40

Seq Identity: 0.24 | Length: 109 / 334

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000132423	coenzyme Q3, methyltransferase

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	13	7	26
disruptive	2	19	17	38
missense	2	19	17	33

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	4	34	15	24
disruptive	6	47	37	70
missense	3	40	34	56

PlasmoDB Total SNPs: 73

Non-coding: 59 | Synonymous: 7 | Nonsynonymous: 5 | Stop Codon: 2

Protein Information

Protein Length: 334 | Molecular Weight (kDa): 40.629

UniProt ID(s): Q8IBK9

PDB ID(s): None

Isoelectric Point: 9.44

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR010233	Ubiquinone biosynthesis O-methyltransferase
InterPro	IPR029063	S-adenosyl-L-methionine-dependent methyltransferase
PFam	PF13489	N/A
Superfamily	SSF53335	S-adenosyl-L-methionine-dependent methyltransferase