Genome location: Pf3D7_07_v3:1,023,600..1,024,634(+)
Genome classification: Core
Product Description: mitochondrial import inner membrane translocase subunit TIM14, putative
SignalP Peptide: MWPVVMLLFGGGVLFVKK
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0001405 | PAM complex, Tim23 associated import motor |
| Component | GO:0005739 | mitochondrion |
| Function | GO:0001671 | ATPase activator activity |
| Function | GO:0051082 | unfolded protein binding |
| Process | GO:0030150 | protein import into mitochondrial matrix |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.18 | 0.91 |
| Trophozoite | expressed | 0.17 | 0.89 |
| Schizont | expressed | 0.05 | 0.96 |
| Gametocyte | possibly expressed | 0.16 | 0.90 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0724400
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS *tentative
MIS: 0.177 | MFS: -3.869 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IBK8
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101286
Most Similar Human Ortholog: A0A2R8Y839
TM-align score: 0.79 | RMSD: 0.48
Seq Identity: 0.74 | Length: 27 / 115
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000120675 | DnaJ heat shock protein family (Hsp40) member C15 |
| ENSG00000205981 | DnaJ heat shock protein family (Hsp40) member C19 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
No passing variants found
PlasmoDB Total SNPs: 86
Non-coding: 76 | Synonymous: 9 | Nonsynonymous: 1 | Stop Codon: 0
Protein Length: 115 | Molecular Weight (kDa): 13.052
UniProt ID(s): Q8IBK8
PDB ID(s): None
Isoelectric Point: 10.83
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001623 | DnaJ domain |
| InterPro | IPR036869 | Chaperone J-domain superfamily |
| PFam | N/A | N/A |
| Superfamily | SSF46565 | Chaperone J-domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |
| 32105215 | Homologue replacement in the import motor of the mitochondrial inner membrane oftrypanosomes | von Kanel C, Munoz-Gomez SA, ..., Schneider A | 10.7554/eLife.52560 |
| 22298705 | Genetic analysis of complex interactions among components of the mitochondrialimport motor and translocon in Saccharomyces cerevisiae | Schilke BA, Hayashi M, Craig EA | 10.1534/genetics.112.138743 |
| 21245414 | Ancient gene duplication provided a key molecular step for anaerobic growth ofBaker's yeast | Hayashi M, Schilke B, ..., Craig EA | 10.1093/molbev/msr019 |