PF3D7_0724800

Genome location: Pf3D7_07_v3:1,046,357..1,049,418(-)

Genome classification: Core

Function and Localization

Product Description: kelch domain-containing protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0003779	actin binding
Process	GO:0007010	cytoskeleton organization

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.27	0.89
Trophozoite	expressed	0.25	0.84
Schizont	expressed	0.27	0.84
Gametocyte	expressed	0.43	0.75

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0724800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.13 | #Insertions: 2

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Not tested (Pb ortholog: PBANKA_0622300)

Modification: Other | RMgm-418

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IBK3

"Best" AlphaFill ligand hit: FUM (fumaric acid, Local RMSD=0.07) with 7C5E (Global RMSD=2.85)

No associated EC numbers

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
Q14145	Kelch-like ECH-associated protein 1		OrthoDB	US10144731, Example 1US10144731, Example 2US10144731, Example 3US10144731, Example 4...

Orthology Information

Ortholog Group (OrthoMCL): OG6_108959

Most Similar Human Ortholog: Q9Y6Y0

TM-align score: 0.58 | RMSD: 2.64

Seq Identity: 0.24 | Length: 269 / 425

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000116679	influenza virus NS1A binding protein

Protein Information

Protein Length: 425 | Molecular Weight (kDa): 48.845

UniProt ID(s): A0A077LPT7, A0A3G2LU59, A0A3G2LUT0, A0A4D7S2U2, A0A5J6C1H9, A0A5J6C1M2, A0A5J6C1S3, A0A5J6C2C2, A0A5J6C2F1, A0A5J6C2Y0, A0A5J6C301, A0A5J6C3H7, A0A5J6C3T7, A0A5J6C3V7, A0A5J6C460, A0A5J6C470, A0A5J6C4K5, A0A5J6C5P9, A0A5J6C6T4, Q8IBK3

PDB ID(s): None

Isoelectric Point: 8.44

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR006652	Kelch repeat type 1
InterPro	IPR015915	Kelch-type beta propeller
PFam	PF01344	Kelch repeat type 1
Superfamily	SSF117281	Kelch-type beta propeller

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	18	11	27
disruptive	13	25	16	51
missense	6	18	15	35

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	9	44	11	24
disruptive	25	63	50	100
missense	9	44	32	63

PlasmoDB Total SNPs: 133

Non-coding: 105 | Synonymous: 17 | Nonsynonymous: 11 | Stop Codon: 0