PF3D7_0725000

Genome location: Pf3D7_07_v3:1,058,649..1,063,957(-)

Genome classification: Core

Function and Localization

Product Description: exonuclease I, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.1.16.1 (Spleen exonuclease)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005634	nucleus
Function	GO:0008409	5'-3' exonuclease activity
Function	GO:0017108	5'-flap endonuclease activity
Function	GO:0048256	flap endonuclease activity
Process	GO:0006310	DNA recombination
Process	GO:0006298	mismatch repair

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	not expressed	0.08	0.96
Trophozoite	expressed	0.57	0.68
Schizont	expressed	0.57	0.66
Gametocyte	expressed	0.52	0.71

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0725000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.33 | #Insertions: 5

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_0622500)

Relative Growth Rate: 0.76 ± 0.15
Confidence: 5.15

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0622500)

Modification: Disrupted | RMgm-2156

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IBK1

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.1.16.1	spleen exonuclease	ATPKClEDTARNAiheparinarseniteATPgammaSUDP-glucosePentamidineacetylationSP-ATPalphaSSP-CTPalphaS...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101671

Most Similar Human Ortholog: Q5T399

TM-align score: 0.93 | RMSD: 1.37

Seq Identity: 0.32 | Length: 135 / 1347

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000174371	exonuclease 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	11	52	34	61
disruptive	44	146	72	197
missense	37	129	66	165

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	18	104	39	80
disruptive	86	288	139	284
missense	58	242	129	210

PlasmoDB Total SNPs: 298

Non-coding: 145 | Synonymous: 75 | Nonsynonymous: 78 | Stop Codon: 0

Protein Information

Protein Length: 1347 | Molecular Weight (kDa): 157.929

UniProt ID(s): Q8IBK1

PDB ID(s): None

Isoelectric Point: 6.64

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR006084	XPG/Rad2 endonuclease
InterPro	IPR006085	XPG N-terminal
InterPro	IPR006086	XPG-I domain
InterPro	IPR029060	PIN-like domain superfamily
InterPro	IPR032641	Exonuclease 1
InterPro	IPR036279	5'-3' exonuclease, C-terminal domain superfamily
PFam	PF00752	XPG N-terminal
PFam	PF00867	XPG-I domain
Superfamily	SSF47807	5'-3' exonuclease, C-terminal domain superfamily
Superfamily	SSF88723	PIN-like domain superfamily