Genome location: Pf3D7_07_v3:1,099,013..1,103,615(+)
Genome classification: Core
Product Description: serine/threonine protein kinase, FIKK family
SignalP Peptide: N/A
# Transmembrane Domains: 1
EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0044538 | host cell periphery |
| Component | GO:0005634 | nucleus |
| Function | GO:0004674 | protein serine/threonine kinase activity |
| Process | GO:0035556 | intracellular signal transduction |
| Process | GO:0044003 | modulation by symbiont of host process |
| Process | GO:0006468 | protein phosphorylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.10 | 0.95 |
| Trophozoite | expressed | 0.13 | 0.93 |
| Schizont | expressed | 0.02 | 0.99 |
| Gametocyte | expressed | 0.32 | 0.81 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0726200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -0.82 | #Insertions: 10
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1225000)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C0H4P3
"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.73) with 4YSJ (Global RMSD=3.37)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.11.1 | non-specific serine/threonine protein kinase | ATPKClDTTP53DRBEDTATBCAKN-93K252aCKI-7LAALSTAACS... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 9 | 42 | 25 | 43 |
| disruptive | 54 | 148 | 49 | 135 |
| missense | 31 | 120 | 40 | 103 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 10 | 87 | 28 | 49 |
| disruptive | 87 | 219 | 97 | 219 |
| missense | 53 | 174 | 68 | 147 |
PlasmoDB Total SNPs: 332
Non-coding: 184 | Synonymous: 83 | Nonsynonymous: 65 | Stop Codon: 0
Protein Length: 845 | Molecular Weight (kDa): 100.07
UniProt ID(s): C0H4P3
PDB ID(s): None
Isoelectric Point: 8.81
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000719 | Protein kinase domain |
| InterPro | IPR008266 | Tyrosine-protein kinase, active site |
| InterPro | IPR011009 | Protein kinase-like domain superfamily |
| PFam | PF00069 | Protein kinase domain |
| Superfamily | SSF56112 | Protein kinase-like domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 20668526 | Plasmodium falciparum FIKK kinase members target distinct components of theerythrocyte membrane. | Nunes MC, Okada M, ..., Scherf A | 10.1371/journal.pone.0011747 |
| 27777305 | Expansion of Lysine-rich Repeats in Plasmodium Proteins Generates NovelLocalization Sequences That Target the Periphery of the Host Erythrocyte. | Davies HM, Thalassinos K, Osborne AR | 10.1074/jbc.M116.761213 |
| 32284562 | An exported kinase family mediates species-specific erythrocyte remodelling andvirulence in human malaria. | Davies H, Belda H, ..., Treeck M | 10.1038/s41564-020-0702-4 |