PF3D7_0726300 (PMS1)

Genome location: Pf3D7_07_v3:1,103,794..1,109,232(+)

Genome classification: Core

Function and Localization

Product Description: DNA mismatch repair protein PMS1, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0032389	MutLalpha complex
Component	GO:0032300	mismatch repair complex
Component	GO:0005634	nucleus
Function	GO:0005524	ATP binding
Function	GO:0016887	ATP hydrolysis activity
Function	GO:0003677	DNA binding
Function	GO:0003684	damaged DNA binding
Process	GO:0006260	DNA replication
Process	GO:0006298	mismatch repair

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	0.35	0.83
Trophozoite	expressed	0.40	0.76
Schizont	expressed	0.31	0.80
Gametocyte	expressed	0.38	0.79

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0726300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 1 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.141 | MFS: -2.924 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0210400)

Relative Growth Rate: 0.18 ± 0.26
Confidence: 4.11

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IBJ3

"Best" AlphaFill ligand hit: ANP (phosphoaminophosphonic acid-adenylate ester, Local RMSD=0.01) with 3H4L (Global RMSD=7.21)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101943

Most Similar Human Ortholog: P54278

TM-align score: 0.46 | RMSD: 5.32

Seq Identity: 0.32 | Length: 462 / 1330

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000122512	PMS1 homolog 2, mismatch repair system component

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	55	28	69
disruptive	19	118	72	189
missense	19	112	68	171

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	10	108	43	83
disruptive	39	261	114	276
missense	35	223	104	214

PlasmoDB Total SNPs: 201

Non-coding: 73 | Synonymous: 63 | Nonsynonymous: 65 | Stop Codon: 0

Protein Information

Protein Length: 1330 | Molecular Weight (kDa): 156.839

UniProt ID(s): Q8IBJ3

PDB ID(s): None

Isoelectric Point: 5.49

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002099	DNA mismatch repair protein family, N-terminal
InterPro	IPR013507	DNA mismatch repair protein, S5 domain 2-like
InterPro	IPR014762	DNA mismatch repair, conserved site
InterPro	IPR014790	MutL, C-terminal, dimerisation
InterPro	IPR020568	Ribosomal protein S5 domain 2-type fold
InterPro	IPR036890	Histidine kinase/HSP90-like ATPase superfamily
InterPro	IPR037198	MutL, C-terminal domain superfamily
InterPro	IPR038973	DNA mismatch repair protein MutL/Mlh/Pms
PFam	PF01119	DNA mismatch repair protein, S5 domain 2-like
PFam	PF08676	MutL, C-terminal, dimerisation
PFam	PF13589
Superfamily	SSF118116	MutL, C-terminal domain superfamily
Superfamily	SSF54211	Ribosomal protein S5 domain 2-type fold
Superfamily	SSF55874	Histidine kinase/HSP90-like ATPase superfamily