Genome location: Pf3D7_07_v3:1,143,936..1,144,903(-)
Genome classification: Core
Product Description: dolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit OST2, putative
SignalP Peptide: N/A
# Transmembrane Domains: 3
EC Numbers: 2.4.99.18 (Dolichyl-diphosphooligosaccharide--protein glycotransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0008250 | oligosaccharyltransferase complex |
| Process | GO:0006487 | protein N-linked glycosylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | no data | N/A | N/A |
| Ring | no data | 0.00 | 1.00 |
| Trophozoite | no data | 0.00 | 1.00 |
| Schizont | no data | 0.00 | 1.00 |
| Gametocyte | no data | 0.00 | 1.00 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0726800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS *tentative
MIS: 0.143 | MFS: -3.108 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C0H4P5
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.4.99.18 | dolichyl-diphosphooligosaccharide-protein glycotransferase | EDTARNAiDigitoninoctylglucosideN-benzoyl-Asn-Leu-ThrN-octanoyl-Asn-Leu-ThrN-t-butoxycarbonyl-Asn-Leu-ThrArg-Asn-Gly-(Gly-allyl)-Ala-Val-methyl esterArg-Asn-Gly-(Gly-vinyl)-Ala-Val-methyl esterArg-Asn-Gly-(Gly-allylepoxide)-Ala-Val-methyl esterN-dinitrobenzoyl-Arg-Asn-Ala-epoxyethylglycine-Ala-ValArg-Asn-Gly-(Gly-epoxyethylglycine)-Ala-Val-methylester... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_103289
Most Similar Human Ortholog: P61803
TM-align score: 0.85 | RMSD: 1.83
Seq Identity: 0.28 | Length: 105 / 107
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000129562 | defender against cell death 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 6 | 4 | 4 |
| disruptive | 0 | 6 | 4 | 10 |
| missense | 0 | 6 | 4 | 10 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 11 | 1 | 6 |
| disruptive | 1 | 13 | 9 | 18 |
| missense | 1 | 10 | 8 | 14 |
PlasmoDB Total SNPs: 66
Non-coding: 64 | Synonymous: 2 | Nonsynonymous: 0 | Stop Codon: 0
Protein Length: 107 | Molecular Weight (kDa): 12.548
UniProt ID(s): C0H4P5
PDB ID(s): None
Isoelectric Point: 8.93
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003038 | DAD/Ost2 |
| PFam | PF02109 | DAD/Ost2 |
| Superfamily | N/A | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 16420350 | Defects in N-glycosylation induce apoptosis in yeast | Hauptmann P, Riel C, ..., Lehle L | 10.1111/j.1365-2958.2005.04981.x |
| 18955371 | The yeast oligosaccharyltransferase complex can be replaced by STT3 fromLeishmania major | Hese K, Otto C, Routier FH, Lehle L | 10.1093/glycob/cwn118 |
| 22116385 | A synthetic heparan sulfate oligosaccharide library reveals the novel enzymaticaction of D-glucosaminyl 3-O-sulfotransferase-3a | Nguyen TK, Arungundram S, ..., Kuberan B | 10.1039/c1mb05221g |
| 16717427 | Yeast cell death caused by mutation of the OST2 gene encoding the epsilon-subunitof Saccharomyces cerevisiae oligosaccharyltransferase | Sugiura M, Takagi H | 10.1271/bbb.70.1234 |
| 36435935 | Molecular basis for glycan recognition and reaction priming of eukaryoticoligosaccharyltransferase | Ramirez AS, de Capitani M, ..., Locher KP | 10.1038/s41467-022-35067-x |