Genome location: Pf3D7_07_v3:1,144,814..1,147,488(+)
Genome classification: Core
Product Description: mitochondrial import inner membrane translocase subunit TIM50, putative
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005744 | TIM23 mitochondrial import inner membrane translocase complex |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005739 | mitochondrion |
| Component | GO:0005634 | nucleus |
| Function | GO:0004721 | phosphoprotein phosphatase activity |
| Process | GO:0030150 | protein import into mitochondrial matrix |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.09 | 0.96 |
| Trophozoite | expressed | 0.44 | 0.74 |
| Schizont | expressed | 0.06 | 0.96 |
| Gametocyte | expressed | 0.23 | 0.86 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0726900
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.12 | MFS: -2.952 | #Insertions: 0
PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0211000)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0211000)
Modification: Tagged | RMgm-1092
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IBI8
"Best" AlphaFill ligand hit: 1PG (2-(2-{2-[2-(2-methoxy-ethoxy)-ethoxy]-ethoxy}-ethoxy)-ethanol, Local RMSD=0.04) with 3L0B (Global RMSD=2.58)
No associated EC numbersNo evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_102279
Most Similar Human Ortholog: M0R2F8
TM-align score: 0.69 | RMSD: 2.96
Seq Identity: 0.22 | Length: 131 / 519
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000105197 | translocase of inner mitochondrial membrane 50 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 28 | 18 | 39 |
| disruptive | 10 | 57 | 26 | 83 |
| missense | 9 | 55 | 25 | 76 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 7 | 67 | 26 | 32 |
| disruptive | 21 | 123 | 64 | 141 |
| missense | 17 | 112 | 51 | 101 |
PlasmoDB Total SNPs: 136
Non-coding: 101 | Synonymous: 20 | Nonsynonymous: 15 | Stop Codon: 0
Protein Length: 519 | Molecular Weight (kDa): 60.945
UniProt ID(s): Q8IBI8
PDB ID(s): None
Isoelectric Point: 9.8
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004274 | FCP1 homology domain |
| InterPro | IPR027111 | Mitochondrial import inner membrane translocase subunit Tim50 |
| InterPro | IPR036412 | HAD-like superfamily |
| PFam | PF03031 | FCP1 homology domain |
| Superfamily | SSF56784 | HAD-like superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 25011111 | Genome-wide functional analysis of Plasmodium protein phosphatases reveals keyregulators of parasite development and differentiation. | Guttery DS, Poulin B, ..., Tewari R | 10.1016/j.chom.2014.05.020 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |
| 34517757 | Trypanosoma brucei Tim50 Possesses PAP Activity and Plays a Critical Role in CellCycle Regulation and Parasite Infectivity | Tripathi A, Singha UK, ..., Chaudhuri M | 10.1128/mBio.01592-21 |
| 28879236 | Cardiolipin mediates membrane and channel interactions of the mitochondrial TIM23 protein import complex receptor Tim50 | Malhotra K, Modak A, ..., Alder NN | 10.1126/sciadv.1700532 |
| 34360547 | Diverse Functions of Tim50, a Component of the Mitochondrial Inner Membrane Protein Translocase | Chaudhuri M, Tripathi A, Gonzalez FS | 10.3390/ijms22157779 |
| 37748811 | Two domains of Tim50 coordinate translocation of proteins across the two mitochondrial membranes | Genge MG, Roy Chowdhury S, ..., Mokranjac D | 10.26508/lsa.202302122 |
| 30683746 | Primary and Secondary Metabolic Effects of a Key Gene Deletion (DeltaYPL062W) inMetabolically Engineered Terpenoid-Producing Saccharomyces cerevisiae | Chen Y, Wang Y, ..., Yuan Y | 10.1128/AEM.01990-18 |
| 32130909 | Transmembrane Coordination of Preprotein Recognition and Motor Coupling by the Mitochondrial Presequence Receptor Tim50 | Caumont-Sarcos A, Moulin C, ..., Ieva R | 10.1016/j.celrep.2020.02.031 |
| 31391278 | The Cross Talk between TbTim50 and PIP39, Two Aspartate-Based ProteinPhosphatases, Maintains Cellular Homeostasis in Trypanosoma brucei | Tripathi A, Singha UK, ..., Chaudhuri M | 10.1128/mSphere.00353-19 |
| 32074073 | From TOM to the TIM23 complex - handing over of a precursor | Callegari S, Cruz-Zaragoza LD, Rehling P | 10.1515/hsz-2020-0101 |