Genome location: Pf3D7_07_v3:1,159,604..1,162,222(-)
Genome classification: Core
Product Description: DNA (cytosine-5)-methyltransferase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.1.1.37 (DNA (cytosine-5-)-methyltransferase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Function | GO:0003886 | DNA (cytosine-5-)-methyltransferase activity |
| Function | GO:0003677 | DNA binding |
| Process | GO:0006306 | DNA methylation |
| Process | GO:0030488 | tRNA methylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.35 | 0.83 |
| Trophozoite | not expressed | 0.62 | 0.65 |
| Schizont | not expressed | 0.16 | 0.90 |
| Gametocyte | not expressed | 0.51 | 0.73 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0727300
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -2.291 | #Insertions: 6
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0211400)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0211400)
Modification: Disrupted | RMgm-1718
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IBI4
"Best" AlphaFill ligand hit: SAH (s-adenosyl-l-homocysteine, Local RMSD=0.37) with 3QV2 (Global RMSD=14.48)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.1.1.37 | DNA (cytosine-5-)-methyltransferase | KClEDTA2'-dCRG108enolasesperminecurcuminNicotineprocainemiR-9-3pmiR-9-5pNSC 14778... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_103302
Most Similar Human Ortholog: A0A0B4J214
TM-align score: 0.88 | RMSD: 2.09
Seq Identity: 0.36 | Length: 135 / 706
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000107614 | tRNA aspartic acid methyltransferase 1 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 31 | 20 | 41 |
| disruptive | 16 | 104 | 66 | 159 |
| missense | 14 | 100 | 63 | 147 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 11 | 66 | 24 | 51 |
| disruptive | 50 | 217 | 95 | 194 |
| missense | 36 | 192 | 85 | 158 |
PlasmoDB Total SNPs: 99
Non-coding: 32 | Synonymous: 34 | Nonsynonymous: 32 | Stop Codon: 1
Protein Length: 706 | Molecular Weight (kDa): 83.784
UniProt ID(s): Q8IBI4
PDB ID(s): None
Isoelectric Point: 8.55
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001525 | C-5 cytosine methyltransferase |
| InterPro | IPR029063 | S-adenosyl-L-methionine-dependent methyltransferase |
| PFam | PF00145 | C-5 cytosine methyltransferase |
| Superfamily | SSF53335 | S-adenosyl-L-methionine-dependent methyltransferase |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 24331467 | Genome-wide mapping of DNA methylation in the human malaria parasite Plasmodiumfalciparum. | Ponts N, Fu L, ..., Le Roch KG | 10.1016/j.chom.2013.11.007 |
| 34724812 | Malaria Parasite Stress Tolerance Is Regulated by DNMT2-Mediated tRNA CytosineMethylation. | Hammam E, Sinha A, ..., Scherf A | 10.1128/mBio.02558-21 |