PF3D7_0727800

Genome location: Pf3D7_07_v3:1,175,363..1,181,839(+)

Genome classification: Core

Function and Localization

Product Description: cation transporting ATPase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 12

EC Numbers: 3.6.3.12 (Transferred entry: 7.2.2.6)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0016021	integral component of membrane
Component	GO:0016020	membrane
Process	GO:0006874	cellular calcium ion homeostasis

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.32	0.85
Trophozoite	not expressed	1.20	0.41
Schizont	possibly expressed	0.40	0.78
Gametocyte	possibly expressed	0.77	0.59

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0727800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.139 | MFS: -2.826 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0211900)

Relative Growth Rate: 0.10 ± 0.46
Confidence: 1.00

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IBH9

"Best" AlphaFill ligand hit: CE1 (o-dodecanyl octaethylene glycol, Local RMSD=0.31) with 6ZHH (Global RMSD=6.40)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.3.12	K+-transporting ATPase	No BRENDA inhibitors

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101914

Most Similar Human Ortholog: Q9HD20

TM-align score: 0.76 | RMSD: 4.20

Seq Identity: 0.33 | Length: 1005 / 1918

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000105726	ATPase 13A1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	30	122	75	131
disruptive	77	248	125	302
missense	56	215	118	273

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	58	221	97	148
disruptive	124	505	245	460
missense	87	431	205	354

PlasmoDB Total SNPs: 293

Non-coding: 58 | Synonymous: 126 | Nonsynonymous: 107 | Stop Codon: 2

Protein Information

Protein Length: 1918 | Molecular Weight (kDa): 225.077

UniProt ID(s): Q8IBH9

PDB ID(s): None

Isoelectric Point: 8.64

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR008250	P-type ATPase, A domain superfamily
InterPro	IPR018303	P-type ATPase, phosphorylation site
InterPro	IPR023298	P-type ATPase, transmembrane domain superfamily
InterPro	IPR023299	P-type ATPase, cytoplasmic domain N
InterPro	IPR036412	HAD-like superfamily
PFam	N/A	N/A
Superfamily	SSF56784	HAD-like superfamily
Superfamily	SSF81653	P-type ATPase, A domain superfamily
Superfamily	SSF81660	P-type ATPase, cytoplasmic domain N
Superfamily	SSF81665	P-type ATPase, transmembrane domain superfamily