Genome location: Pf3D7_07_v3:1,190,698..1,192,848(-)
Genome classification: Core
Product Description: eukaryotic translation initiation factor 2 subunit alpha
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0033290 | eukaryotic 48S preinitiation complex |
| Component | GO:0005850 | eukaryotic translation initiation factor 2 complex |
| Component | GO:0005851 | eukaryotic translation initiation factor 2B complex |
| Component | GO:0043614 | multi-eIF complex |
| Component | GO:0005634 | nucleus |
| Function | GO:0003723 | RNA binding |
| Function | GO:0043022 | ribosome binding |
| Function | GO:0003743 | translation initiation factor activity |
| Process | GO:0006413 | translational initiation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | expressed | 0.66 | 0.69 |
| Trophozoite | expressed | 0.88 | 0.53 |
| Schizont | expressed | 0.22 | 0.85 |
| Gametocyte | expressed | 0.42 | 0.76 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0728000
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.12 | MFS: -3.124 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IBH7
"Best" AlphaFill ligand hit: HEM (heme, Local RMSD=9.29) with 8C3W (Global RMSD=17.41)
No associated EC numbersOrthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P05198 | Eukaryotic translation initiation factor 2 subunit 1 | OMA, OrthoDB, OrthoMCL BLAST | CHEMBL4862478CHEMBL4852735CHEMBL4870495CHEMBL4866245... |
Ortholog Group (OrthoMCL): OG6_101220
Most Similar Human Ortholog: G3V4T5
TM-align score: 0.83 | RMSD: 2.75
Seq Identity: 0.50 | Length: 265 / 329
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000134001 | eukaryotic translation initiation factor 2 subunit alpha |
Protein Length: 329 | Molecular Weight (kDa): 37.82
UniProt ID(s): Q8IBH7
PDB ID(s): None
Isoelectric Point: 4.9
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR003029 | S1 domain |
| InterPro | IPR011488 | Translation initiation factor 2, alpha subunit |
| InterPro | IPR012340 | Nucleic acid-binding, OB-fold |
| InterPro | IPR022967 | RNA-binding domain, S1 |
| InterPro | IPR024054 | Translation initiation factor 2, alpha subunit, middle domain superfamily |
| InterPro | IPR024055 | Translation initiation factor 2, alpha subunit, C-terminal |
| PFam | PF00575 | S1 domain |
| PFam | PF07541 | Translation initiation factor 2, alpha subunit |
| Superfamily | SSF110993 | Translation initiation factor 2, alpha subunit, C-terminal |
| Superfamily | SSF116742 | Translation initiation factor 2, alpha subunit, middle domain superfamily |
| Superfamily | SSF50249 | Nucleic acid-binding, OB-fold |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 1 | 12 | 15 | 26 |
| disruptive | 1 | 4 | 5 | 11 |
| missense | 1 | 2 | 5 | 7 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 2 | 46 | 12 | 21 |
| disruptive | 1 | 18 | 17 | 61 |
| missense | 1 | 11 | 14 | 38 |
PlasmoDB Total SNPs: 133
Non-coding: 127 | Synonymous: 5 | Nonsynonymous: 1 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 33906926 | Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH | Sinha A, Baumgarten S, ..., Scherf A | 10.1128/mBio.00661-21 |