Genome location: Pf3D7_07_v3:1,246,810..1,248,317(+)
Genome classification: Core
Product Description: 1-cys peroxiredoxin
SignalP Peptide: MRMRRTILIFTVILIPLTFC
# Transmembrane Domains: 1
EC Numbers: 1.11.1.15 (Transferred entry: 1.11.1.24)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005829 | cytosol |
| Function | GO:0016209 | antioxidant activity |
| Function | GO:0008379 | thioredoxin peroxidase activity |
| Process | GO:0045454 | cell redox homeostasis |
| Process | GO:0034599 | cellular response to oxidative stress |
| Process | GO:0042744 | hydrogen peroxide catabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | not expressed | 0.02 | 0.99 |
| Trophozoite | not expressed | 0.18 | 0.88 |
| Schizont | not expressed | 0.03 | 0.98 |
| Gametocyte | not expressed | 0.09 | 0.94 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0729200
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.857 | MFS: -2.976 | #Insertions: 1
PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0213300)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0213300)
Modification: Disrupted | RMgm-1728
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q5MYR6
"Best" AlphaFill ligand hit: MCT (4-methyl-1,2-benzenediol, Local RMSD=0.21) with 4K7N (Global RMSD=3.35)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 1.11.1.15 | peroxiredoxin | No BRENDA inhibitors |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_101112
Most Similar Human Ortholog: P30044
TM-align score: 0.69 | RMSD: 1.83
Seq Identity: 0.28 | Length: 159 / 240
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000126432 | peroxiredoxin 5 |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 14 | 7 | 20 |
| disruptive | 5 | 8 | 9 | 21 |
| missense | 5 | 8 | 9 | 13 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 31 | 10 | 12 |
| disruptive | 6 | 35 | 20 | 49 |
| missense | 6 | 25 | 14 | 25 |
PlasmoDB Total SNPs: 54
Non-coding: 38 | Synonymous: 11 | Nonsynonymous: 5 | Stop Codon: 0
Protein Length: 240 | Molecular Weight (kDa): 28.124
UniProt ID(s): Q5MYR6
Isoelectric Point: 7.76
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR013740 | Redoxin |
| InterPro | IPR036249 | Thioredoxin-like superfamily |
| InterPro | IPR037944 | Peroxiredoxin-5-like |
| PFam | PF08534 | Redoxin |
| Superfamily | SSF52833 | Thioredoxin-like superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 15701514 | Crystal structure of a novel Plasmodium falciparum 1-Cys peroxiredoxin. | Sarma GN, Nickel C, ..., Karplus PA | 10.1016/j.jmb.2004.12.022 |
| 21203490 | Compartmentation of redox metabolism in malaria parasites. | Kehr S, Sturm N, ..., Becker K | 10.1371/journal.ppat.1001242 |
| 28357258 | Prokaryotic ancestry and gene fusion of a dual localized peroxiredoxin in malariaparasites. | Djuika CF, Huerta-Cepas J, ..., Deponte M | 10.15698/mic2015.01.182 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |