About Gene List

PF3D7_0729500 (MT-A70)

Genome location: Pf3D7_07_v3:1,254,778..1,258,355(+)

Genome classification: Core

Function and Localization

Product Description: N6-adenosine-methyltransferase MT-A70

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.1.1.348 (mRNA m(6)A methyltransferase);2.1.1.62 (mRNA (2'-O-methyladenosine-N(6)-)-methyltransferase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0036396 RNA N6-methyladenosine methyltransferase complex
Component GO:0005737 cytoplasm
Component GO:0016607 nuclear speck
Component GO:0005634 nucleus
Function GO:0016422 mRNA (2'-O-methyladenosine-N6-)-methyltransferase activity
Function GO:0001734 mRNA (N6-adenosine)-methyltransferase activity
Function GO:0008168 methyltransferase activity
Function GO:0005515 protein binding
Process GO:0001510 RNA methylation
Process GO:0009451 RNA modification
Process GO:0080009 mRNA methylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite expressed N/A N/A
Ring expressed 0.52 0.74
Trophozoite expressed 0.53 0.70
Schizont expressed 0.22 0.86
Gametocyte expressed 0.54 0.69

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0729500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.126 | MFS: -3.166 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_0213600)

  • Relative Growth Rate: 0.15 ± 0.20
  • Confidence: 4.63

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I6Z2

"Best" AlphaFill ligand hit: ADN (adenosine, Local RMSD=0.40) with 6TTP (Global RMSD=0.92)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
2.1.1.348 mRNA m6A methyltransferase sinefunginS-adenosyl-L-homocysteine
2.1.1.62 mRNA (2'-O-methyladenosine-N6-)-methyltransferase N-[[3-(4-methylphenyl)adamant-1-yl]carbamoyl]-L-phenylalanine

Orthology to BindingDB Entries:

UniProt Protein Name Species Homology Source BindingDB Ligands
Q9HCE5 N6-adenosine-methyltransferase catalytic/non-catalytic subunit OrthoDB, OrthoMCL BLAST US11725010, Example 1US11725010, Example 47US11725010, Example 3US11725010, Example 4...
Q86U44 N6-adenosine-methyltransferase catalytic subunit OrthoDB, OrthoMCL BLAST CHEMBL4854665CHEMBL4850503CHEMBL4872977CHEMBL4856985...

Orthology Information

Ortholog Group (OrthoMCL): OG6_103808

Most Similar Human Ortholog: Q86U44

TM-align score: 0.57 | RMSD: 4.70

Seq Identity: 0.46 | Length: 389 / 760

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000165819 methyltransferase like 3

Protein Information

Protein Length: 760 | Molecular Weight (kDa): 89.635

UniProt ID(s): Q8I6Z2

PDB ID(s): None

Isoelectric Point: 9.15

Protein Domain Annotations:

Source Family ID Description
InterPro IPR007757 MT-A70-like
InterPro IPR029063 S-adenosyl-L-methionine-dependent methyltransferase
PFam PF05063 MT-A70-like
Superfamily SSF53335 S-adenosyl-L-methionine-dependent methyltransferase

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 9 48 22 45
disruptive 21 62 45 91
missense 15 61 45 81

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 16 86 30 37
disruptive 39 143 53 122
missense 28 131 48 85

PlasmoDB Total SNPs: 161

Non-coding: 105 | Synonymous: 32 | Nonsynonymous: 24 | Stop Codon: 0

Associated Publications

PMID Title Authors DOI/Link
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104
31384004 Transcriptome-wide dynamics of extensive m(6)A mRNA methylation during Plasmodiumfalciparum blood-stage development. Baumgarten S, Bryant JM, ..., Scherf A 10.1038/s41564-019-0521-7
31722409 A distinct class of eukaryotic MT-A70 methyltransferases maintain symmetric DNAN6-adenine methylation at the ApT dinucleotides as an epigenetic mark associatedwith transcription Wang Y, Sheng Y, ..., Gao S 10.1093/nar/gkz1053
12384598 Induction of sporulation in Saccharomyces cerevisiae leads to the formation ofN6-methyladenosine in mRNA: a potential mechanism for the activity of the IME4gene Clancy MJ, Shambaugh ME, Timpte CS, Bokar JA 10.1093/nar/gkf573