PF3D7_0730100

Genome location: Pf3D7_07_v3:1,282,562..1,285,940(+)

Genome classification: Core

Function and Localization

Product Description: tRNA pseudouridine synthase D, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 5.4.99.12 (tRNA pseudouridine(38-40) synthase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005634	nucleus
Function	GO:0009982	pseudouridine synthase activity
Process	GO:0001522	pseudouridine synthesis

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.07	0.97
Trophozoite	not expressed	0.29	0.84
Schizont	not expressed	0.06	0.96
Gametocyte	not expressed	0.32	0.81

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0730100

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.292 | #Insertions: 3

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0214200)

Relative Growth Rate: 1.02 ± 0.12
Confidence: 5.60

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0214200)

Modification: Disrupted | RMgm-1732

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IBF9

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
5.4.99.12	tRNA pseudouridine38-40 synthase	tRNApoly(rA)npoly(rC)npoly(rG)npoly(rI)npoly(rU)niodoacetateCalf thymus DNADithionitrobenzoate5-fluorouracil tRNAPhep-chloromercuribenzoatefluorouracil-substituted tRNA

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533184

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	11	36	13	38
disruptive	22	97	54	134
missense	20	91	52	127

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	17	58	21	63
disruptive	33	210	108	227
missense	28	184	93	179

PlasmoDB Total SNPs: 132

Non-coding: 78 | Synonymous: 34 | Nonsynonymous: 20 | Stop Codon: 0

Protein Information

Protein Length: 868 | Molecular Weight (kDa): 104.216

UniProt ID(s): Q8IBF9

PDB ID(s): None

Isoelectric Point: 9.85

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001656	Pseudouridine synthase, TruD
InterPro	IPR020103	Pseudouridine synthase, catalytic domain superfamily
PFam	PF01142	Pseudouridine synthase, TruD
Superfamily	SSF55120	Pseudouridine synthase, catalytic domain superfamily