About Gene List

PF3D7_0730300 (ApiAP2)

Genome location: Pf3D7_07_v3:1,297,144..1,302,090(+)

Genome classification: Core

Function and Localization

Product Description: AP2 domain transcription factor AP2-L, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005634 nucleus

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite expressed N/A N/A
Ring expressed 1.94 0.29
Trophozoite expressed 1.15 0.45
Schizont expressed 0.50 0.76
Gametocyte possibly expressed 0.42 0.77

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0730300

Resistome Missense Mutations: K1199Q, H1298Q

Resistome Compounds with Missense Mutations: Artemisinin, MMV665794

Resistome # Samples with Disruptive Mutations: 2 (2 missense, 1 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -2.478 | #Insertions: 1

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Not different from wild type (Pb ortholog: PBANKA_0214400)

Modification: Disrupted | RMgm-4115

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IBF6

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533374

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 14 65 27 68
disruptive 51 155 72 185
missense 41 124 60 153

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 34 108 40 75
disruptive 95 338 165 395
missense 69 264 131 239

PlasmoDB Total SNPs: 269

Non-coding: 142 | Synonymous: 70 | Nonsynonymous: 57 | Stop Codon: 0

Protein Information

Protein Length: 1331 | Molecular Weight (kDa): 155.132

UniProt ID(s): Q8IBF6

PDB ID(s): None

Isoelectric Point: 4.71

Protein Domain Annotations:

Source Family ID Description
InterPro IPR001471 AP2/ERF domain
InterPro IPR028078 AP2-coincident, C-terminal domain
PFam PF00847 AP2/ERF domain
PFam PF14733 AP2-coincident, C-terminal domain
Superfamily N/A N/A

Associated Publications

PMID Title Authors DOI/Link
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104
23144823 Identification of an AP2-family protein that is critical for malaria liver stagedevelopment. Iwanaga S, Kaneko I, Kato T, Yuda M 10.1371/journal.pone.0047557
34492224 Single-genome sequencing reveals within-host evolution of human malariaparasites Dia A, Jett C, ..., Cheeseman IH 10.1016/j.chom.2021.08.009
37898053 Cell cycle-regulated ApiAP2s and parasite development: the Toxoplasma paradigm Zarringhalam K, Ye S, ..., Gubbels MJ 10.1016/j.mib.2023.102383
35087229 The transcriptional regulator HDP1 controls expansion of the inner membranecomplex during early sexual differentiation of malaria parasites Campelo Morillo RA, Tong X, ..., Kafsack BFC 10.1038/s41564-021-01045-0
35871979 A transcription factor helps Plasmodium falciparum gametocytogenesis take shape McLean KJ, Niles JC 10.1016/j.pt.2022.07.004
38311493 Sex in a dish: high-efficiency Toxoplasma pre-sexual development Hryckowian ND, Knoll LJ 10.1016/j.pt.2024.01.009
36292619 Epigenetic and Epitranscriptomic Gene Regulation in Plasmodium falciparum and HowWe Can Use It against Malaria Serrano-Duran R, Lopez-Farfan D, Gomez-Diaz E 10.3390/genes13101734
37350586 An apicomplexan bromodomain protein, TgBDP1, associates with diverse epigeneticfactors to regulate essential transcriptional processes in Toxoplasma gondii Fleck K, McNutt S, Chu F, Jeffers V 10.1128/mbio.03573-22
34880324 Essential role of a Plasmodium berghei heat shock protein (PBANKA_0938300) ingametocyte development Kashif M, Quadiri A, Singh AP 10.1038/s41598-021-03059-4
36847501 Single-Cell Transcriptomics To Define Plasmodium falciparum Stage Transition inthe Mosquito Midgut Mohammed M, Dziedziech A, ..., Ankarklev J 10.1128/spectrum.03671-22
38370614 Structural characterization of the ACDC domain from ApiAP2 proteins of themalaria parasite Le Berre M, Tubiana T, ..., Nessler S 10.1101/2024.02.09.579679
35083215 PfAP2-EXP2, an Essential Transcription Factor for the IntraerythrocyticDevelopment of Plasmodium falciparum Shang X, Wang C, ..., Jiang M 10.3389/fcell.2021.782293
34874774 The Extracellular Milieu of Toxoplasma's Lytic Cycle Drives Lab Adaptation,Primarily by Transcriptional Reprogramming Primo VA Jr, Rezvani Y, ..., Gubbels MJ 10.1128/mSystems.01196-21
37293082 PfAP2-MRP DNA-binding protein is a master regulator of parasite pathogenesisduring malaria parasite blood stages Subudhi AK, Green JL, ..., Pain A 10.1101/2023.05.23.541898
36223427 Inhibitors of ApiAP2 protein DNA binding exhibit multistage activity againstPlasmodium parasites Russell TJ, De Silva EK, ..., Llinas M 10.1371/journal.ppat.1010887
35288749 Genome-wide landscape of ApiAP2 transcription factors reveals aheterochromatin-associated regulatory network during Plasmodium falciparumblood-stage development Shang X, Wang C, ..., Zhang Q 10.1093/nar/gkac176
34400833 A heat-shock response regulated by the PfAP2-HS transcription factor protectshuman malaria parasites from febrile temperatures Tinto-Font E, Michel-Todo L, ..., Cortes A 10.1038/s41564-021-00940-w