Genome location: Pf3D7_07_v3:1,366,407..1,370,816(+)
Genome classification: Core
Product Description: acyl-CoA synthetase
SignalP Peptide: MNISFTVCSLFILLINVVLHG
# Transmembrane Domains: 0
EC Numbers: 6.2.1.3 (Long-chain-fatty-acid--CoA ligase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020011 | apicoplast |
| Component | GO:0005783 | endoplasmic reticulum |
| Component | GO:0020030 | infected host cell surface knob |
| Component | GO:0016020 | membrane |
| Component | GO:0005634 | nucleus |
| Function | GO:0016208 | AMP binding |
| Function | GO:0004467 | long-chain fatty acid-CoA ligase activity |
| Process | GO:0006631 | fatty acid metabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | possibly expressed | N/A | N/A |
| Ring | expressed | 0.29 | 0.87 |
| Trophozoite | expressed | 1.49 | 0.34 |
| Schizont | expressed | 0.37 | 0.80 |
| Gametocyte | possibly expressed | 0.06 | 0.97 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0731600
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -2.618 | #Insertions: 2
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1453300)
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8I6Z1
"Best" AlphaFill ligand hit: DYD ((2s,5s)-hexane-2,5-diol, Local RMSD=0.26) with 6Q2M (Global RMSD=3.84)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.2.1.3 | long-chain-fatty-acid-CoA ligase | ATPAMPNEMSDSEDTAsaltsoleateGW1929Brij 58Tween 80naproxentriacsin... |
No evidence of orthology to BindingDB entries
Ortholog Group (OrthoMCL): OG6_100152
Most Similar Human Ortholog: B7Z3Z9
TM-align score: 0.91 | RMSD: 2.53
Seq Identity: 0.23 | Length: 515 / 811
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000151726 | acyl-CoA synthetase long chain family member 1 |
| ENSG00000164398 | acyl-CoA synthetase long chain family member 6 |
| ENSG00000197142 | acyl-CoA synthetase long chain family member 5 |
| ENSG00000281938 | novel transcript |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 0 | 30 | 17 | 20 |
| disruptive | 15 | 26 | 20 | 36 |
| missense | 15 | 25 | 16 | 33 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 14 | 36 | 18 | 27 |
| disruptive | 25 | 65 | 38 | 122 |
| missense | 20 | 54 | 32 | 81 |
PlasmoDB Total SNPs: 165
Non-coding: 118 | Synonymous: 29 | Nonsynonymous: 18 | Stop Codon: 0
Protein Length: 811 | Molecular Weight (kDa): 93.284
UniProt ID(s): Q8I6Z1
PDB ID(s): None
Isoelectric Point: 9.34
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000873 | AMP-dependent synthetase/ligase |
| InterPro | IPR020845 | AMP-binding, conserved site |
| PFam | PF00501 | AMP-dependent synthetase/ligase |
| Superfamily | SSF56801 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 28981210 | Proteome and Structural Organization of the Knob Complex on the Surface of thePlasmodium Infected Red Blood Cell. | Alampalli SV, Grover M, ..., Acharya P | 10.1002/prca.201600177 |
| 30212465 | Integrative proteomics and bioinformatic prediction enable a high-confidenceapicoplast proteome in malaria parasites. | Boucher MJ, Ghosh S, ..., Yeh E | 10.1371/journal.pbio.2005895 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 36358951 | Bio-Multifunctional Sponges Containing Alginate/Chitosan/SargassumPolysaccharides Promote the Healing of Full-Thickness Wounds | Quan W, Li P, ..., Ouyang Q | 10.3390/biom12111601 |