Genome location: Pf3D7_08_v3:29,147..39,682(-)
Genome classification: SubtelomericHypervariable
Product Description: erythrocyte membrane protein 1, PfEMP1
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: None
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0020002 | host cell plasma membrane |
| Component | GO:0020030 | infected host cell surface knob |
| Function | GO:0050839 | cell adhesion molecule binding |
| Function | GO:0005515 | protein binding |
| Process | GO:0020033 | antigenic variation |
| Process | GO:0098609 | cell-cell adhesion |
| Process | GO:0020035 | cytoadherence to microvasculature, mediated by symbiont protein |
| Process | GO:0020013 | modulation by symbiont of host erythrocyte aggregation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | not expressed | N/A | N/A |
| Ring | possibly expressed | 0.01 | 0.99 |
| Trophozoite | possibly expressed | 0.01 | 0.99 |
| Schizont | not expressed | 0.04 | 0.97 |
| Gametocyte | possibly expressed | 0.02 | 0.98 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0800200
Resistome Missense Mutations: K318R, T330I, T330P, T330S, I418T, E523K, G884S
Resistome Compounds with Missense Mutations: AN13762, MMV665928
Resistome # Samples with Disruptive Mutations: 4 (3 missense, 3 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: -0.128 | #Insertions: 4
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: N/A
"Best" AlphaFill ligand hit: No AlphaFill hits
No associated EC numbersNo evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
No passing variants found
PlasmoDB Total SNPs: 3527
Non-coding: 611 | Synonymous: 675 | Nonsynonymous: 2220 | Stop Codon: 21
Protein Length: 2858 | Molecular Weight (kDa): 330.697
UniProt ID(s): A0A191VZ63, A0A5P1A018, A0A5P1A041, A0A5P1A113, A0A5P1A134, A0A5P1A1K3, A0A5P1A1N4, A0A5P1A294, A0A5P1A2H3, A0A5P1A2I5, A0A5P1A2L9, A0A5P1A2M5, A3R6V3, B7T1P0, I1X0L2, I1X0L4, I2CIW9, Q8IAK2
PDB ID(s): None
Isoelectric Point: 7.52
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR004258 | Duffy-binding-like domain |
| InterPro | IPR008602 | Duffy-antigen binding |
| InterPro | IPR029210 | Plasmodium falciparum erythrocyte membrane protein-1, N-terminal segment |
| InterPro | IPR029211 | Plasmodium falciparum erythrocyte membrane protein 1, acidic terminal segment |
| InterPro | IPR041480 | Cysteine-rich interdomain region 1 gamma |
| PFam | PF03011 | Duffy-binding-like domain |
| PFam | PF05424 | Duffy-antigen binding |
| PFam | PF15445 | Plasmodium falciparum erythrocyte membrane protein 1, acidic terminal segment |
| PFam | PF15447 | Plasmodium falciparum erythrocyte membrane protein-1, N-terminal segment |
| PFam | PF18562 | Cysteine-rich interdomain region 1 gamma |
| Superfamily | SSF140924 | N/A |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 9230440 | P. falciparum rosetting mediated by a parasite-variant erythrocyte membraneprotein and complement-receptor 1. | Rowe JA, Moulds JM, Newbold CI, Miller LH | 10.1038/40888 |
| 9291676 | Knob proteins in falciparum malaria. | Sharma YD | https://pubmed.ncbi.nlm.nih.gov/9291676/ |
| 9786187 | A recombinant peptide based on PfEMP-1 blocks and reverses adhesion ofmalaria-infected red blood cells to CD36 under flow. | Cooke BM, Nicoll CL, Baruch DI, Coppel RL | 10.1046/j.1365-2958.1998.01040.x |
| 11316367 | Variant antigens of Plasmodium falciparum encoded by the var multigenic familyare multifunctional macromolecules. | Nogueira PA, Wunderlich G, Pereira da Silva LH | 10.1016/s0923-2508(01)01184-6 |
| 11420100 | Molecules on the surface of the Plasmodium falciparum infected erythrocyte andtheir role in malaria pathogenesis and immune evasion. | Craig A, Scherf A | https://pubmed.ncbi.nlm.nih.gov/11420100/ |
| 18773118 | The cysteine-rich interdomain region from the highly variable plasmodiumfalciparum erythrocyte membrane protein-1 exhibits a conserved structure. | Klein MM, Gittis AG, ..., Garboczi DN | 10.1371/journal.ppat.1000147 |
| 18785843 | Antigenic variation in Plasmodium falciparum. | Scherf A, Lopez-Rubio JJ, Riviere L | 10.1146/annurev.micro.61.080706.093134 |
| 24983468 | A Plasmodium falciparum PHIST protein binds the virulence factor PfEMP1 andcomigrates to knobs on the host cell surface. | Oberli A, Slater LM, ..., Vakonakis I | 10.1096/fj.14-256057 |
| 28461509 | Evidence of strain structure in Plasmodium falciparum var gene repertoires inchildren from Gabon, West Africa. | Day KP, Artzy-Randrup Y, ..., Pascual M | 10.1073/pnas.1613018114 |
| 30576479 | Plasmodium falciparum protein ' PfJ23' hosts distinct binding sites for majorvirulence factor ' PfEMP1' and Maurer's cleft marker ' PfSBP1' | Kaur J, Kumar V, ..., Hora R | 10.1093/femspd/fty090 |