PF3D7_0801500 (NOL10)

Genome location: Pf3D7_08_v3:106,068..108,513(-)

Genome classification: Core

Function and Localization

Product Description: nucleolar protein 10, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005730	nucleolus
Component	GO:0032040	small-subunit processome
Process	GO:0000462	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.74	0.65
Trophozoite	expressed	0.58	0.68
Schizont	possibly expressed	0.12	0.93
Gametocyte	possibly expressed	0.30	0.82

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0801500

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.258 | MFS: -2.597 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IAL3

"Best" AlphaFill ligand hit: ALQ (2-methyl-propionic acid, Local RMSD=0.69) with 4GM3 (Global RMSD=3.83)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102419

Most Similar Human Ortholog: Q9BSC4

TM-align score: 0.63 | RMSD: 2.35

Seq Identity: 0.38 | Length: 362 / 549

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000115761	nucleolar protein 10

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	10	29	13	34
disruptive	8	38	26	67
missense	8	37	24	63

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	14	51	23	32
disruptive	16	102	72	124
missense	11	90	61	87

PlasmoDB Total SNPs: 122

Non-coding: 98 | Synonymous: 13 | Nonsynonymous: 11 | Stop Codon: 0

Protein Information

Protein Length: 549 | Molecular Weight (kDa): 64.409

UniProt ID(s): Q8IAL3

PDB ID(s): None

Isoelectric Point: 7

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR036322	WD40-repeat-containing domain superfamily
InterPro	IPR040382	Nucleolar protein 10/Enp2
PFam	N/A	N/A
Superfamily	SSF50978	WD40-repeat-containing domain superfamily