PF3D7_0801800

Genome location: Pf3D7_08_v3:117,932..122,572(-)

Genome classification: Core

Function and Localization

Product Description: mannose-6-phosphate isomerase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: 5.3.1.8 (Mannose-6-phosphate isomerase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005829	cytosol
Component	GO:0005634	nucleus
Function	GO:0004476	mannose-6-phosphate isomerase activity
Function	GO:0008270	zinc ion binding
Process	GO:0009298	GDP-mannose biosynthetic process
Process	GO:0000032	cell wall mannoprotein biosynthetic process
Process	GO:0006486	protein glycosylation

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	1.69	0.33
Trophozoite	expressed	2.07	0.16
Schizont	expressed	0.93	0.53
Gametocyte	possibly expressed	1.11	0.45

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0801800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.999 | MFS: -2.624 | #Insertions: 1

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1228400)

Relative Growth Rate: 0.61 ± 0.13
Confidence: 5.45

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1228400)

Modification: Disrupted | RMgm-3313

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IAL6

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
5.3.1.8	mannose-6-phosphate isomerase	EDTAPCMBHgCl2cysteinephosphateGDP-mannoseADP-glucoseGDP-D-mannosedithiothreitol2,2'-dipyridylL-ascorbic acid2,3-Butanedione...

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
P34948	Mannose-6-phosphate isomerase	Candida albicans	OrthoDB, OrthoMCL	CHEMBL75413
P34949	Mannose-6-phosphate isomerase	Homo sapiens	OrthoDB	med.21724, Compound 153MLS000027386MLS000055225MLS000054052...

Orthology Information

Ortholog Group (OrthoMCL): OG6_102059

Most Similar Human Ortholog: F5GX71

TM-align score: 0.87 | RMSD: 2.65

Seq Identity: 0.21 | Length: 359 / 1011

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000178802	mannose phosphate isomerase

Protein Information

Protein Length: 1011 | Molecular Weight (kDa): 117.662

UniProt ID(s): Q8IAL6

PDB ID(s): None

Isoelectric Point: 7.12

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001250	Mannose-6-phosphate isomerase, type I
InterPro	IPR011051	RmlC-like cupin domain superfamily
InterPro	IPR016305	Mannose-6-phosphate isomerase
PFam	PF01238	Mannose-6-phosphate isomerase, type I
Superfamily	SSF51182	RmlC-like cupin domain superfamily

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	14	70	47	70
disruptive	21	110	64	133
missense	7	82	55	117

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	24	135	47	78
disruptive	60	255	97	262
missense	18	198	79	198

PlasmoDB Total SNPs: 183

Non-coding: 106 | Synonymous: 57 | Nonsynonymous: 20 | Stop Codon: 0