PF3D7_0802300 (PWP2)

Genome location: Pf3D7_08_v3:166,711..170,995(-)

Genome classification: Core

Function and Localization

Product Description: periodic tryptophan protein 2, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0034388	Pwp2p-containing subcomplex of 90S preribosome
Component	GO:0005730	nucleolus
Component	GO:0032040	small-subunit processome
Process	GO:0000462	maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA)
Process	GO:0000028	ribosomal small subunit assembly

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	0.46	0.78
Trophozoite	expressed	0.44	0.76
Schizont	expressed	0.09	0.94
Gametocyte	expressed	0.30	0.82

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0802300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.119 | MFS: -3.078 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IAM3

"Best" AlphaFill ligand hit: FAR (farnesyl, Local RMSD=3.34) with 1A0R (Global RMSD=12.53)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102497

Most Similar Human Ortholog: Q15269

TM-align score: 0.86 | RMSD: 2.77

Seq Identity: 0.28 | Length: 832 / 1121

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000241945	PWP2 small subunit processome component
ENSG00000275464	periodic tryptophan protein 2 homolog

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	16	58	32	73
disruptive	34	75	58	137
missense	23	64	52	117

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	29	116	56	70
disruptive	51	191	105	226
missense	30	148	85	161

PlasmoDB Total SNPs: 174

Non-coding: 74 | Synonymous: 65 | Nonsynonymous: 35 | Stop Codon: 0

Protein Information

Protein Length: 1121 | Molecular Weight (kDa): 129.052

UniProt ID(s): Q8IAM3

PDB ID(s): None

Isoelectric Point: 8.01

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001680	WD40 repeat
InterPro	IPR007148	Small-subunit processome, Utp12
InterPro	IPR011047	Quinoprotein alcohol dehydrogenase-like superfamily
InterPro	IPR027145	Periodic tryptophan protein 2
InterPro	IPR036322	WD40-repeat-containing domain superfamily
PFam	PF00400	WD40 repeat
PFam	PF04003	Small-subunit processome, Utp12
Superfamily	SSF50978	WD40-repeat-containing domain superfamily
Superfamily	SSF50998	Quinoprotein alcohol dehydrogenase-like superfamily