About Gene List

PF3D7_0803000 (CYP81)

Genome location: Pf3D7_08_v3:196,270..198,711(+)

Genome classification: Core

Function and Localization

Product Description: peptidyl-prolyl cis-trans isomerase

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 5.2.1.8 (Peptidylprolyl isomerase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0000974 Prp19 complex
Component GO:0071013 catalytic step 2 spliceosome
Component GO:0005634 nucleus
Component GO:0071014 post-mRNA release spliceosomal complex

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite possibly expressed N/A N/A
Ring possibly expressed 0.48 0.77
Trophozoite expressed 0.67 0.62
Schizont expressed 0.30 0.80
Gametocyte possibly expressed 0.75 0.59

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0803000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.999 | MFS: -2.903 | #Insertions: 2

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IAN0

"Best" AlphaFill ligand hit: O4B (1,4,7,10,13,16-hexaoxacyclooctadecane, Local RMSD=0.03) with 6R8W (Global RMSD=1.48)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
5.2.1.8 peptidylprolyl isomerase ATPNEMSDSPCMBFK506QAEGPKAla-ProjugloneSer-ProGPI-1046RNase T1Rapamycin...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533341

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 5 25 19 38
disruptive 32 73 53 137
missense 18 59 48 112

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 9 59 37 39
disruptive 59 194 111 213
missense 29 152 85 131

PlasmoDB Total SNPs: 107

Non-coding: 35 | Synonymous: 44 | Nonsynonymous: 28 | Stop Codon: 0

Protein Information

Protein Length: 677 | Molecular Weight (kDa): 80.923

UniProt ID(s): Q8IAN0

PDB ID(s): None

Isoelectric Point: 9.76

Protein Domain Annotations:

Source Family ID Description
InterPro IPR002130 Cyclophilin-type peptidyl-prolyl cis-trans isomerase domain
InterPro IPR013260 mRNA splicing factor SYF2
InterPro IPR029000 Cyclophilin-like domain superfamily
PFam PF00160 Cyclophilin-type peptidyl-prolyl cis-trans isomerase domain
PFam PF08231 mRNA splicing factor SYF2
Superfamily SSF50891 Cyclophilin-like domain superfamily

Associated Publications

PMID Title Authors DOI/Link
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104
23181666 Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. Oehring SC, Woodcroft BJ, ..., Voss TS 10.1186/gb-2012-13-11-r108
30474973 A Combinatorial Approach To Study Cytochrome P450 Enzymes for De Novo Productionof Steviol Glucosides in Baker's Yeast Gold ND, Fossati E, ..., Martin VJJ 10.1021/acssynbio.8b00470