About Gene List

PF3D7_0803400 (RAD54)

Genome location: Pf3D7_08_v3:211,217..217,167(+)

Genome classification: Core

Function and Localization

Product Description: DNA repair and recombination protein RAD54, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.4.12 (DNA helicase)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0005634 nucleus
Function GO:0015616 DNA translocase activity
Function GO:0003684 damaged DNA binding
Process GO:0006310 DNA recombination
Process GO:0000724 double-strand break repair via homologous recombination
Process GO:0045003 double-strand break repair via synthesis-dependent strand annealing
Process GO:0007131 reciprocal meiotic recombination

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite expressed N/A N/A
Ring expressed 0.06 0.97
Trophozoite expressed 0.55 0.68
Schizont expressed 0.54 0.69
Gametocyte expressed 1.10 0.48

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0803400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -0.7 | #Insertions: 4

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1226800)

  • Relative Growth Rate: 0.78 ± 0.19
  • Confidence: 4.66

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1226800)

Modification: Disrupted | RMgm-3306

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IAN4

"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.69) with 7EPU (Global RMSD=8.04)

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
3.6.4.12 DNA helicase No BRENDA inhibitors

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102456

Most Similar Human Ortholog: A0A087WW72

TM-align score: 0.89 | RMSD: 1.35

Seq Identity: 0.39 | Length: 75 / 1239

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000085999 RAD54 like

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 11 63 26 89
disruptive 58 114 59 184
missense 26 84 47 141

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 20 125 63 87
disruptive 87 234 111 306
missense 45 169 79 218

PlasmoDB Total SNPs: 302

Non-coding: 218 | Synonymous: 49 | Nonsynonymous: 35 | Stop Codon: 0

Protein Information

Protein Length: 1239 | Molecular Weight (kDa): 143.045

UniProt ID(s): Q25868, Q8IAN4

PDB ID(s): None

Isoelectric Point: 5.43

Protein Domain Annotations:

Source Family ID Description
InterPro IPR000330 SNF2-related, N-terminal domain
InterPro IPR001650 Helicase, C-terminal
InterPro IPR014001 Helicase superfamily 1/2, ATP-binding domain
InterPro IPR027417 P-loop containing nucleoside triphosphate hydrolase
PFam PF00176 SNF2-related, N-terminal domain
PFam PF00271 Helicase, C-terminal
Superfamily SSF52540 P-loop containing nucleoside triphosphate hydrolase

Associated Publications

PMID Title Authors DOI/Link
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104
23631919 Opposing roles for two molecular forms of replication protein A inRad51-Rad54-mediated DNA recombination in Plasmodium falciparum. Gopalakrishnan AM, Kumar N 10.1128/mBio.02515-14
37393982 Deficiency in homologous recombination is associated with changes in cell cyclingand morphology in Saccharomyces cerevisiae Holland CL, Weis MF, ..., Lewis LK 10.1016/j.yexcr.2023.113701
38002960 Homologous Recombination and Repair Functions Required for Mutagenicity duringYeast Meiosis Morciano L, Elgrabli RM, Zenvirth D, Arbel-Eden A 10.3390/genes14112017
36508468 Rad51-mediated interhomolog recombination during budding yeast meiosis ispromoted by the meiotic recombination checkpoint and the conserved Pif1 helicase Ziesel A, Weng Q, ..., Hollingsworth NM 10.1371/journal.pgen.1010407