PF3D7_0803500

Genome location: Pf3D7_08_v3:217,476..222,589(+)

Genome classification: Core

Function and Localization

Product Description: AAA family ATPase, putative

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005739	mitochondrion
Function	GO:0004176	ATP-dependent peptidase activity
Process	GO:0006508	proteolysis

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	0.49	0.82
Trophozoite	expressed	0.35	0.79
Schizont	expressed	0.59	0.75
Gametocyte	not expressed	0.28	0.84

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0803500

Resistome Missense Mutations: D1339Y

Resistome Compounds with Missense Mutations: MMV665794

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.144 | MFS: -2.885 | #Insertions: 0

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1226700)

Relative Growth Rate: 0.50 ± 0.16
Confidence: 5.06

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1226700)

Modification: Disrupted | RMgm-3305

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IAN5

"Best" AlphaFill ligand hit: SKE (4-({5-amino-1-[(2,6-difluorophenyl)carbonyl]-1h-1,2,4-triazol-3-yl}amino)benzenesulfonamide, Local RMSD=0.40) with 6P11 (Global RMSD=9.31)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_532999

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	15	62	35	81
disruptive	97	178	105	240
missense	43	103	77	181

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	34	116	44	96
disruptive	164	387	170	379
missense	68	255	126	232

PlasmoDB Total SNPs: 263

Non-coding: 50 | Synonymous: 129 | Nonsynonymous: 84 | Stop Codon: 0

Protein Information

Protein Length: 1467 | Molecular Weight (kDa): 175.575

UniProt ID(s): Q8IAN5

PDB ID(s): None

Isoelectric Point: 6.8

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR003593	AAA+ ATPase domain
InterPro	IPR003959	ATPase, AAA-type, core
InterPro	IPR025662	Sigma-54 interaction domain, ATP-binding site 1
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF00004	ATPase, AAA-type, core
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase