PF3D7_0803600

Genome location: Pf3D7_08_v3:222,976..230,577(+)

Genome classification: Core

Function and Localization

Product Description: GRAM domain-containing protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 2

EC Numbers: None

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	possibly expressed	nd	nd
Trophozoite	expressed	nd	nd
Schizont	expressed	nd	nd
Gametocyte	possibly expressed	nd	nd

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0803600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 2 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -2.897 | #Insertions: 5

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_0800300)

Relative Growth Rate: 0.94 ± 0.13
Confidence: 5.50

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_0800300)

Modification: Disrupted | RMgm-2283

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: A0A146M1U1

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101929

Most Similar Human Ortholog: A0A0J9YXF6

TM-align score: 0.58 | RMSD: 2.93

Seq Identity: 0.24 | Length: 111 / 1599

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000023171	GRAM domain containing 1B
ENSG00000089351	GRAM domain containing 1A

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	14	72	46	80
disruptive	72	230	112	305
missense	41	186	96	254

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	32	135	59	99
disruptive	132	458	211	451
missense	76	354	161	320

PlasmoDB Total SNPs: 597

Non-coding: 358 | Synonymous: 130 | Nonsynonymous: 108 | Stop Codon: 1

Protein Information

Protein Length: 1599 | Molecular Weight (kDa): 190.847

UniProt ID(s): A0A146M1U1

PDB ID(s): None

Isoelectric Point: 8.75

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR004182	GRAM domain
InterPro	IPR040147	Membrane-anchored lipid-binding protein Ysp2/Lam4-like
PFam	PF02893	GRAM domain
Superfamily	N/A	N/A