PF3D7_0803700 (g-tub)

Genome location: Pf3D7_08_v3:230,967..233,374(+)

Genome classification: Core

Function and Localization

Product Description: tubulin gamma chain

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005813	centrosome
Component	GO:0005737	cytoplasm
Component	GO:0000930	gamma-tubulin complex
Component	GO:0005874	microtubule
Component	GO:0005815	microtubule organizing center
Component	GO:0005634	nucleus
Component	GO:0005819	spindle
Function	GO:0005525	GTP binding
Function	GO:0003723	RNA binding
Function	GO:0005200	structural constituent of cytoskeleton
Process	GO:0000212	meiotic spindle organization
Process	GO:0000226	microtubule cytoskeleton organization
Process	GO:0007020	microtubule nucleation
Process	GO:0007017	microtubule-based process
Process	GO:0000278	mitotic cell cycle
Process	GO:0000070	mitotic sister chromatid segregation
Process	GO:0007052	mitotic spindle organization

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	possibly expressed	N/A	N/A
Ring	expressed	0.02	0.99
Trophozoite	expressed	0.13	0.91
Schizont	expressed	0.03	0.97
Gametocyte	expressed	0.17	0.89

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0803700

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.142 | MFS: -2.886 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IAN7

"Best" AlphaFill ligand hit: GTP (guanosine-5'-triphosphate, Local RMSD=0.09) with 4X1K (Global RMSD=2.54)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101897

Most Similar Human Ortholog: P23258

TM-align score: 0.97 | RMSD: 1.25

Seq Identity: 0.66 | Length: 446 / 452

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000037042	tubulin gamma 2
ENSG00000131462	tubulin gamma 1

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	8	21	15	15
disruptive	0	2	1	13
missense	0	2	0	10

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	12	44	11	14
disruptive	1	7	12	60
missense	0	4	8	39

PlasmoDB Total SNPs: 125

Non-coding: 108 | Synonymous: 16 | Nonsynonymous: 0 | Stop Codon: 1

Protein Information

Protein Length: 452 | Molecular Weight (kDa): 51.637

UniProt ID(s): Q8IAN7

PDB ID(s): None

Isoelectric Point: 6.43

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000217	Tubulin
InterPro	IPR002454	Gamma tubulin
InterPro	IPR003008	Tubulin/FtsZ, GTPase domain
InterPro	IPR008280	Tubulin/FtsZ, C-terminal
InterPro	IPR017975	Tubulin, conserved site
InterPro	IPR018316	Tubulin/FtsZ, 2-layer sandwich domain
InterPro	IPR036525	Tubulin/FtsZ, GTPase domain superfamily
PFam	PF00091	Tubulin/FtsZ, GTPase domain
PFam	PF03953	Tubulin/FtsZ, 2-layer sandwich domain
Superfamily	SSF52490	Tubulin/FtsZ, GTPase domain superfamily
Superfamily	SSF55307	Tubulin/FtsZ, C-terminal

PMID	Title	Authors	DOI/Link
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
23181666	Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum.	Oehring SC, Woodcroft BJ, ..., Voss TS	10.1186/gb-2012-13-11-r108
33906926	Functional Characterization of the m(6)A-Dependent Translational Modulator PfYTH	Sinha A, Baumgarten S, ..., Scherf A	10.1128/mBio.00661-21