Product Description: proteasome subunit beta type-4
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 3.4.25.1 (Proteasome endopeptidase complex)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005634 | nucleus |
| Component | GO:0005839 | proteasome core complex |
| Function | GO:0004175 | endopeptidase activity |
| Process | GO:0010498 | proteasomal protein catabolic process |
| Process | GO:0010499 | proteasomal ubiquitin-independent protein catabolic process |
| Process | GO:0043161 | proteasome-mediated ubiquitin-dependent protein catabolic process |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.68 | 0.69 |
| Trophozoite | expressed | 1.50 | 0.31 |
| Schizont | expressed | 1.03 | 0.50 |
| Gametocyte | expressed | 1.01 | 0.47 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0803800
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.227 | MFS: -2.689 | #Insertions: 0
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1226500)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1226500)
Modification: Disrupted | RMgm-3304
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q7K6A9
"Best" AlphaFill ligand hit: No AlphaFill hits
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.25.1 | proteasome endopeptidase complex | ATPSDSPSIEDTANH4+EGTATLCKALLNNMDANLVSPI 1PA28... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P28070 | Proteasome subunit beta type-4 | OMA, OrthoDB | CHEMBL4877708 |
Ortholog Group (OrthoMCL): OG6_101718
Most Similar Human Ortholog: P28070
TM-align score: 0.79 | RMSD: 1.40
Seq Identity: 0.39 | Length: 218 / 265
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000159377 | proteasome 20S subunit beta 4 |
Protein Length: 265 | Molecular Weight (kDa): 30.872
PDB ID(s): 5FMG
Isoelectric Point: 6.39
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001353 | Proteasome, subunit alpha/beta |
| InterPro | IPR016050 | Proteasome beta-type subunit, conserved site |
| InterPro | IPR016295 | Proteasome subunit beta 4 |
| InterPro | IPR029055 | Nucleophile aminohydrolases, N-terminal |
| PFam | PF00227 | Proteasome, subunit alpha/beta |
| Superfamily | SSF56235 | Nucleophile aminohydrolases, N-terminal |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 4 | 11 | 4 | 16 |
| disruptive | 0 | 8 | 3 | 17 |
| missense | 0 | 8 | 3 | 16 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 5 | 21 | 8 | 18 |
| disruptive | 2 | 19 | 14 | 46 |
| missense | 2 | 18 | 13 | 36 |
PlasmoDB Total SNPs: 102
Non-coding: 87 | Synonymous: 14 | Nonsynonymous: 1 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |
| 31384003 | The structure of the PA28-20S proteasome complex from Plasmodium falciparum andimplications for proteostasis. | Xie SC, Metcalfe RD, ..., Tilley L | 10.1038/s41564-019-0524-4 |