PF3D7_0804000

Genome location: Pf3D7_08_v3:236,954..241,286(+)

Genome classification: Core

Function and Localization

Product Description: cactin homolog, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0005681	spliceosomal complex
Process	GO:0045292	mRNA cis splicing, via spliceosome
Process	GO:0010468	regulation of gene expression

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.78	0.64
Trophozoite	expressed	0.78	0.57
Schizont	expressed	0.38	0.76
Gametocyte	possibly expressed	0.78	0.58

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0804000

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.182 | MFS: -2.77 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IAN8

"Best" AlphaFill ligand hit: DCP (2'-deoxycytidine-5'-triphosphate, Local RMSD=0.45) with 4F4W (Global RMSD=14.13)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102945

Most Similar Human Ortholog: K7EIU6

TM-align score: 0.97 | RMSD: 0.77

Seq Identity: 0.55 | Length: 117 / 802

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000105298	cactin, spliceosome C complex subunit

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	3	31	13	44
disruptive	37	88	41	118
missense	15	70	32	94

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	9	62	18	48
disruptive	61	177	107	202
missense	24	125	77	126

PlasmoDB Total SNPs: 311

Non-coding: 219 | Synonymous: 45 | Nonsynonymous: 46 | Stop Codon: 1

Protein Information

Protein Length: 802 | Molecular Weight (kDa): 97.412

UniProt ID(s): Q8IAN8

PDB ID(s): None

Isoelectric Point: 9.34

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR018816	Cactin, central domain
InterPro	IPR019134	Cactin, C-terminal
PFam	PF09732	Cactin, C-terminal
PFam	PF10312	Cactin, central domain
Superfamily	N/A	N/A