PF3D7_0804400 (METAP1c)

Genome location: Pf3D7_08_v3:245,027..248,440(+)

Genome classification: Core

Function and Localization

Product Description: methionine aminopeptidase 1c, putative

SignalP Peptide: MNISIFFFLFLYSGSICA

# Transmembrane Domains: 0

EC Numbers: 3.4.11.18 (Methionyl aminopeptidase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0020011	apicoplast
Function	GO:0003729	mRNA binding
Function	GO:0070006	metalloaminopeptidase activity
Function	GO:0008235	metalloexopeptidase activity
Process	GO:0006508	proteolysis

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.09	0.96
Trophozoite	possibly expressed	0.39	0.76
Schizont	possibly expressed	0.19	0.88
Gametocyte	not expressed	0.21	0.88

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0804400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.177 | MFS: -2.788 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1226200)

Relative Growth Rate: 0.10 ± nan
Confidence: 1.00

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IAP0

"Best" AlphaFill ligand hit: HED (2-hydroxyethyl disulfide, Local RMSD=0.34) with 4IDY (Global RMSD=0.78)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.4.11.18	methionyl aminopeptidase	BRENDA SOAP query unsuccessful

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_171502

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	7	24	19	37
disruptive	26	89	39	104
missense	16	63	29	78

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	12	51	29	49
disruptive	61	164	73	152
missense	25	119	59	94

PlasmoDB Total SNPs: 185

Non-coding: 83 | Synonymous: 65 | Nonsynonymous: 37 | Stop Codon: 0

Protein Information

Protein Length: 740 | Molecular Weight (kDa): 88.179

UniProt ID(s): Q8IAP0

PDB ID(s): None

Isoelectric Point: 9.33

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000994	Peptidase M24
InterPro	IPR001714	Peptidase M24, methionine aminopeptidase
InterPro	IPR036005	Creatinase/aminopeptidase-like
PFam	PF00557	Peptidase M24
Superfamily	SSF55920	Creatinase/aminopeptidase-like