PF3D7_0804800 (CYP24)

Genome location: Pf3D7_08_v3:278,082..279,206(+)

Genome classification: Core

Function and Localization

Product Description: peptidyl-prolyl cis-trans isomerase

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 5.2.1.8 (Peptidylprolyl isomerase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0043231	intracellular membrane-bounded organelle
Function	GO:0016018	cyclosporin A binding
Function	GO:0003755	peptidyl-prolyl cis-trans isomerase activity
Process	GO:0006457	protein folding
Process	GO:0000413	protein peptidyl-prolyl isomerization

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.50	0.76
Trophozoite	expressed	0.56	0.67
Schizont	expressed	0.10	0.93
Gametocyte	expressed	0.28	0.83

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0804800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.849 | MFS: -2.289 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1225800)

Relative Growth Rate: 0.48 ± 0.46
Confidence: 2.92

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8I6S4

"Best" AlphaFill ligand hit: LSA (saccharin, Local RMSD=0.07) with 4N1O (Global RMSD=1.64)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
5.2.1.8	peptidylprolyl isomerase	ATPNEMSDSPCMBFK506QAEGPKAla-ProjugloneSer-ProGPI-1046RNase T1Rapamycin...

Orthology to BindingDB Entries:

UniProt	Protein Name	Species	Homology Source	BindingDB Ligands
P10111	Peptidyl-prolyl cis-trans isomerase A	Rattus norvegicus	HOGENOM	CHEMBL13611CHEMBL441409CHEMBL13778CHEMBL13768...
P62937	Peptidyl-prolyl cis-trans isomerase A	Homo sapiens	OMA	Cyclophilin Inhibitor, 15Aryl 1-indanylketone, 1Aryl 1-indanylketone, 2Aryl 1-indanylketone, 3...
Q08752	Peptidyl-prolyl cis-trans isomerase D	Homo sapiens	OrthoDB	Aryl 1-indanylketone, 1Aryl 1-indanylketone, 2US8901295, F428US8901295, F671...
P23284	Peptidyl-prolyl cis-trans isomerase B	Homo sapiens	OrthoDB	US8901295, F607Aryl 1-indanylketone, 3Aryl 1-indanylketone, 4US8901295, F428...
P45877	Peptidyl-prolyl cis-trans isomerase C	Homo sapiens	OrthoDB	CHEMBL2424822

Orthology Information

Ortholog Group (OrthoMCL): OG6_103291

Most Similar Human Ortholog: C9JQD4

TM-align score: 0.99 | RMSD: 0.54

Seq Identity: 0.62 | Length: 151 / 217

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000171960	peptidylprolyl isomerase H

Protein Information

Protein Length: 217 | Molecular Weight (kDa): 24.892

UniProt ID(s): Q27716, Q8I6S4, Q9U6U5

PDB ID(s): None

Isoelectric Point: 6.74

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002130	Cyclophilin-type peptidyl-prolyl cis-trans isomerase domain
InterPro	IPR020892	Cyclophilin-type peptidyl-prolyl cis-trans isomerase, conserved site
InterPro	IPR029000	Cyclophilin-like domain superfamily
PFam	PF00160	Cyclophilin-type peptidyl-prolyl cis-trans isomerase domain
Superfamily	SSF50891	Cyclophilin-like domain superfamily

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	1	8	9	13
disruptive	0	6	8	28
missense	0	5	7	22

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	2	27	10	7
disruptive	4	26	28	62
missense	2	22	26	36

PlasmoDB Total SNPs: 46

Non-coding: 45 | Synonymous: 1 | Nonsynonymous: 0 | Stop Codon: 0