PF3D7_0804900

Genome location: Pf3D7_08_v3:279,251..282,015(-)

Genome classification: Core

Function and Localization

Product Description: GTPase-activating protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005634	nucleus
Process	GO:0048205	COPI coating of Golgi vesicle

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.64	0.69
Trophozoite	expressed	0.46	0.73
Schizont	expressed	0.58	0.64
Gametocyte	expressed	0.47	0.73

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0804900

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.989 | MFS: -2.411 | #Insertions: 1

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1225700)

Relative Growth Rate: 0.13 ± 0.07
Confidence: 6.64

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IAP4

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101784

Most Similar Human Ortholog: E9PMR9

TM-align score: 0.94 | RMSD: 0.89

Seq Identity: 0.45 | Length: 87 / 491

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000149182	ADP ribosylation factor GTPase activating protein 2
ENSG00000242247	ADP ribosylation factor GTPase activating protein 3

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	5	21	11	14
disruptive	33	56	26	67
missense	15	44	25	52

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	11	34	18	22
disruptive	47	117	40	111
missense	24	90	34	79

PlasmoDB Total SNPs: 162

Non-coding: 99 | Synonymous: 48 | Nonsynonymous: 15 | Stop Codon: 0

Protein Information

Protein Length: 491 | Molecular Weight (kDa): 56.501

UniProt ID(s): Q8IAP4

PDB ID(s): None

Isoelectric Point: 7.66

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001164	Arf GTPase activating protein
InterPro	IPR037278	ARFGAP/RecO-like zinc finger
PFam	PF01412	Arf GTPase activating protein
Superfamily	SSF57863	ARFGAP/RecO-like zinc finger

PMID	Title	Authors	DOI/Link
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
23181666	Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum.	Oehring SC, Woodcroft BJ, ..., Voss TS	10.1186/gb-2012-13-11-r108