PF3D7_0805400

Genome location: Pf3D7_08_v3:303,060..304,788(-)

Genome classification: Core

Function and Localization

Product Description: N-acetyltransferase, GNAT family, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.3.1.- (Transferring groups other than amino-acyl groups.);2.3.1.1 (Amino-acid N-acetyltransferase);2.3.1.88 (Transferred entry: 2.3.1.254, 2.3.1.255, 2.3.1.256, 2.3.1.257, 2.3.1.258and 2.3.1.259)

Curated GO (PlasmoDB): None

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	no data	N/A	N/A
Ring	no data	0.16	0.92
Trophozoite	no data	0.16	0.90
Schizont	no data	0.05	0.97
Gametocyte	no data	0.13	0.92

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0805400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 1 | MFS: -1.92 | #Insertions: 2

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1225200)

Relative Growth Rate: 0.96 ± 0.10
Confidence: 6.01

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1225200)

Modification: Disrupted | RMgm-3297

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H4R5

"Best" AlphaFill ligand hit: CMC (carboxymethyl coenzyme *a, Local RMSD=0.05) with 6Z00 (Global RMSD=2.84)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.3.1.-	Ex: [myelin-proteolipid] O-palmitoyltransferase	CoAKClArgATPADPEDTAMgCl2CaCl2MnCl2AgNO3CoCl2FeSO4...
2.3.1.1	amino-acid N-acetyltransferase	CoAKClArgEDTAMgCl2CaCl2MnCl2AgNO3CoCl2FeSO4FeCl3BaCl2...
2.3.1.88	peptide alpha-N-acetyltransferase	No BRENDA inhibitors

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_101962

Most Similar Human Ortholog: B4DK34

TM-align score: 0.91 | RMSD: 1.38

Seq Identity: 0.41 | Length: 103 / 225

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000139977	N-alpha-acetyltransferase 30, NatC catalytic subunit

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	0	7	3	16
disruptive	1	15	7	20
missense	1	15	7	15

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	1	16	14	11
disruptive	6	33	16	57
missense	4	30	10	41

PlasmoDB Total SNPs: 62

Non-coding: 49 | Synonymous: 12 | Nonsynonymous: 1 | Stop Codon: 0

Protein Information

Protein Length: 225 | Molecular Weight (kDa): 26.304

UniProt ID(s): C0H4R5

PDB ID(s): None

Isoelectric Point: 4.78

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000182	GNAT domain
InterPro	IPR016181	Acyl-CoA N-acyltransferase
PFam	PF00583	GNAT domain
Superfamily	SSF55729	Acyl-CoA N-acyltransferase