Product Description: serine/threonine protein kinase, FIKK family
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 2.7.11.1 (Non-specific serine/threonine protein kinase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005737 | cytoplasm |
| Component | GO:0005829 | cytosol |
| Component | GO:0005634 | nucleus |
| Function | GO:0004674 | protein serine/threonine kinase activity |
| Process | GO:0035556 | intracellular signal transduction |
| Process | GO:0006468 | protein phosphorylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | no data | N/A | N/A |
| Ring | no data | 0.08 | 0.96 |
| Trophozoite | no data | 0.67 | 0.65 |
| Schizont | no data | 1.21 | 0.45 |
| Gametocyte | no data | 0.34 | 0.80 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0805700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 0.998 | MFS: -2.681 | #Insertions: 1
PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1225000)
RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1225000)
Modification: Disrupted | RMgm-3295
More info: PhenoPlasm Link
AlphaFill Uniprot ID: C0H4R8
"Best" AlphaFill ligand hit: IPH (phenol, Local RMSD=0.05) with 6Q7S (Global RMSD=8.26)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 2.7.11.1 | non-specific serine/threonine protein kinase | ATPKClDTTP53DRBEDTATBCAKN-93K252aCKI-7LAALSTAACS... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 8 | 67 | 29 | 60 |
| disruptive | 45 | 170 | 72 | 181 |
| missense | 19 | 130 | 56 | 149 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 22 | 111 | 49 | 82 |
| disruptive | 84 | 302 | 167 | 340 |
| missense | 40 | 226 | 142 | 236 |
PlasmoDB Total SNPs: 381
Non-coding: 223 | Synonymous: 111 | Nonsynonymous: 47 | Stop Codon: 0
Protein Length: 1457 | Molecular Weight (kDa): 171.805
UniProt ID(s): C0H4R8
PDB ID(s): None
Isoelectric Point: 6.42
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR000719 | Protein kinase domain |
| InterPro | IPR008266 | Tyrosine-protein kinase, active site |
| InterPro | IPR011009 | Protein kinase-like domain superfamily |
| PFam | PF00069 | Protein kinase domain |
| Superfamily | SSF56112 | Protein kinase-like domain superfamily |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 26112892 | Biochemical characterization of FIKK8--A unique protein kinase from the malariaparasite Plasmodium falciparum and other apicomplexans. | Osman KT, Lou HJ, ..., Hui R | 10.1016/j.molbiopara.2015.06.002 |
| 32284562 | An exported kinase family mediates species-specific erythrocyte remodelling andvirulence in human malaria | Davies H, Belda H, ..., Treeck M | 10.1038/s41564-020-0702-4 |