PF3D7_0806300 (FLP)

Genome location: Pf3D7_08_v3:337,844..343,424(-)

Genome classification: Core

Function and Localization

Product Description: ferlin-like protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 1

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0071944	cell periphery
Component	GO:0031410	cytoplasmic vesicle
Process	GO:0035891	exit from host cell
Process	GO:0007009	plasma membrane organization

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.03	0.98
Trophozoite	expressed	0.20	0.89
Schizont	expressed	0.71	0.60
Gametocyte	expressed	0.26	0.85

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0806300

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 2 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.156 | MFS: -2.87 | #Insertions: 0

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1224400)

Relative Growth Rate: 0.06 ± 0.10
Confidence: 6.00

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1224400)

Modification: Tagged | RMgm-4578

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H4S2

"Best" AlphaFill ligand hit: 1PG (2-(2-{2-[2-(2-methoxy-ethoxy)-ethoxy]-ethoxy}-ethoxy)-ethanol, Local RMSD=0.41) with 7TX9 (Global RMSD=10.53)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_116719

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	16	53	49	84
disruptive	26	56	33	94
missense	18	42	28	76

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	30	130	54	79
disruptive	42	119	75	203
missense	29	79	56	145

PlasmoDB Total SNPs: 162

Non-coding: 59 | Synonymous: 79 | Nonsynonymous: 24 | Stop Codon: 0

Protein Information

Protein Length: 1704 | Molecular Weight (kDa): 197.634

UniProt ID(s): C0H4S2

PDB ID(s): None

Isoelectric Point: 6.13

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR000008	C2 domain
InterPro	IPR012968	FerIin domain
InterPro	IPR037721	Ferlin family
InterPro	IPR037724	Ferlin, fifth C2 domain
PFam	PF00168	C2 domain
Superfamily	SSF49562	N/A

PMID	Title	Authors	DOI/Link
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
30597708	Transmission of the malaria parasite requires ferlin for gamete egress from thered blood cell.	Obrova K, Cyrklaff M, ..., Mueller AK	10.1111/cmi.12999