PF3D7_0806400 (ALG13)

Genome location: Pf3D7_08_v3:344,038..345,365(+)

Genome classification: Core

Function and Localization

Product Description: UDP-N-acetylglucosamine transferase subunit ALG13, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 2.4.1.141 (N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase);2.4.1.227 (Undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Function	GO:0004577	N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase activity
Process	GO:0006488	dolichol-linked oligosaccharide biosynthetic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.02	0.99
Trophozoite	expressed	0.24	0.85
Schizont	expressed	0.36	0.78
Gametocyte	not expressed	0.17	0.90

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0806400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.191 | MFS: -3.406 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: Not tested (Pb ortholog: PBANKA_1224300)

Modification: Other | RMgm-474

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IAQ4

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
2.4.1.141	N-acetylglucosaminyldiphosphodolichol N-acetylglucosaminyltransferase	UDPUTPUMPEDTADiumycinUDPglucosebacitracinAmphomycinShowdomycin5-fluoro N-acetylglucosamineN-acetyl-D-glucosaminyl-diphosphodolicholP1-dolichyl P2-(2-O-ethyl-alpha-D-glucopyranosyl) diphosphate...
2.4.1.227	undecaprenyldiphospho-muramoylpentapeptide beta-N-acetylglucosaminyltransferase	UDPEDTAnisinToXa-1PyDT-1methanolmurgocilPolyoxin DVancomycinMoenomycinramoplanintunicamycin...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102820

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

No passing variants found

PlasmoDB Total SNPs: 85

Non-coding: 70 | Synonymous: 14 | Nonsynonymous: 1 | Stop Codon: 0

Protein Information

Protein Length: 177 | Molecular Weight (kDa): 20.8

UniProt ID(s): Q8IAQ4

PDB ID(s): None

Isoelectric Point: 8.6

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR007235	Glycosyl transferase, family 28, C-terminal
InterPro	IPR039042	UDP-N-acetylglucosamine transferase subunit Alg13-like
PFam	PF04101	Glycosyl transferase, family 28, C-terminal
Superfamily	SSF53756	N/A