PF3D7_0806600

Genome location: Pf3D7_08_v3:349,042..353,536(-)

Genome classification: Core

Function and Localization

Product Description: kinesin-like protein, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005871	kinesin complex
Component	GO:0005874	microtubule
Function	GO:0016887	ATP hydrolysis activity
Function	GO:0008017	microtubule binding
Function	GO:0003777	microtubule motor activity
Process	GO:0007018	microtubule-based movement

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.24	0.88
Trophozoite	not expressed	0.14	0.91
Schizont	not expressed	0.08	0.94
Gametocyte	not expressed	0.55	0.70

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0806600

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 1 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.991 | MFS: -2.747 | #Insertions: 1

PlasmoGEM Phenotype: Slow (Pb ortholog: PBANKA_1224100)

Relative Growth Rate: 0.75 ± 0.12
Confidence: 5.67

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1224100)

Modification: Disrupted | RMgm-3292

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H4S3

"Best" AlphaFill ligand hit: ADP (adenosine-5'-diphosphate, Local RMSD=0.09) with 4ZHI (Global RMSD=6.35)

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_533673

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	10	32	22	64
disruptive	34	88	67	131
missense	21	72	52	105

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	12	70	43	60
disruptive	61	175	113	207
missense	37	125	92	128

PlasmoDB Total SNPs: 217

Non-coding: 153 | Synonymous: 38 | Nonsynonymous: 26 | Stop Codon: 0

Protein Information

Protein Length: 943 | Molecular Weight (kDa): 110.876

UniProt ID(s): C0H4S3, Q2KNR2, Q2KNR4

PDB ID(s): None

Isoelectric Point: 9.26

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001752	Kinesin motor domain
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
InterPro	IPR027640	Kinesin-like protein
PFam	PF00225	Kinesin motor domain
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase