About Gene List

PF3D7_0806800

Genome location: Pf3D7_08_v3:362,926..367,241(-)

Genome classification: Core

Function and Localization

Product Description: V-type proton ATPase subunit a, putative

SignalP Peptide: N/A

# Transmembrane Domains: 6

EC Numbers: 3.6.3.14 (Transferred entry: 7.1.2.2)

Curated GO (PlasmoDB):

Type GO Term Name
Component GO:0020020 food vacuole
Component GO:0016020 membrane
Component GO:0016469 proton-transporting two-sector ATPase complex
Component GO:0016471 vacuolar proton-transporting V-type ATPase complex
Function GO:0051117 ATPase binding
Function GO:0009055 electron transfer activity
Function GO:0046933 proton-transporting ATP synthase activity, rotational mechanism
Function GO:0046961 proton-transporting ATPase activity, rotational mechanism
Process GO:1902600 proton transmembrane transport
Process GO:0007035 vacuolar acidification

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage LR class MCA mean MCA prop. zeros
Sporozoite expressed N/A N/A
Ring expressed 1.77 0.31
Trophozoite expressed 1.15 0.42
Schizont expressed 1.55 0.27
Gametocyte expressed 1.10 0.44

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0806800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.149 | MFS: -2.715 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IAQ8

"Best" AlphaFill ligand hit: No AlphaFill hits

BRENDA EC Inhibitors:

EC # Name EC Inhibitors
3.6.3.14 H+-transporting two-sector ATPase No BRENDA inhibitors

Orthology to BindingDB Entries:

UniProt Protein Name Species Homology Source BindingDB Ligands
Q9I8D0 V-type proton ATPase 116 kDa subunit a 1 Gallus gallus OrthoMCL CHEMBL449696CHEMBL291117CHEMBL39485CHEMBL41588...

Orthology Information

Ortholog Group (OrthoMCL): OG6_100301

Most Similar Human Ortholog: Q9HBG4

TM-align score: 0.90 | RMSD: 3.11

Seq Identity: 0.31 | Length: 808 / 1053

All Human Orthologs (OrthoMCL):

Gene ID Description
ENSG00000033627 ATPase H+ transporting V0 subunit a1
ENSG00000105929 ATPase H+ transporting V0 subunit a4
ENSG00000110719 T cell immune regulator 1, ATPase H+ transporting V0 subunit a3
ENSG00000185344 ATPase H+ transporting V0 subunit a2

Protein Information

Protein Length: 1053 | Molecular Weight (kDa): 122.999

UniProt ID(s): Q2KNV7, Q8IAQ8

PDB ID(s): None

Isoelectric Point: 7.09

Protein Domain Annotations:

Source Family ID Description
InterPro IPR002490 V-type ATPase, V0 complex, 116kDa subunit family
PFam PF01496 V-type ATPase, V0 complex, 116kDa subunit family
Superfamily N/A N/A

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type common rare doubleton singleton
synonymous 11 44 33 70
disruptive 18 34 31 71
missense 8 28 25 58

Any inclusion in genotype call

variant type common rare doubleton singleton
synonymous 20 114 47 63
disruptive 34 106 58 181
missense 14 73 50 130

PlasmoDB Total SNPs: 180

Non-coding: 117 | Synonymous: 43 | Nonsynonymous: 20 | Stop Codon: 0

Associated Publications

PMID Title Authors DOI/Link
11152613 Predicting transmembrane protein topology with a hidden Markov model: applicationto complete genomes. Krogh A, Larsson B, von Heijne G, Sonnhammer EL 10.1006/jmbi.2000.4315
16267556 A protein interaction network of the malaria parasite Plasmodium falciparum. LaCount DJ, Vignali M, ..., Hughes RE 10.1038/nature04104