PF3D7_0807100 (PSH3)

Genome location: Pf3D7_08_v3:372,356..376,952(-)

Genome classification: Core

Function and Localization

Product Description: DNA helicase PSH3

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: 3.6.4.12 (DNA helicase);3.6.4.13 (RNA helicase)

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005737	cytoplasm
Component	GO:0005634	nucleus
Function	GO:0043138	3'-5' DNA helicase activity
Function	GO:0016887	ATP hydrolysis activity
Function	GO:0003723	RNA binding
Function	GO:0003724	RNA helicase activity
Function	GO:0017116	single-stranded DNA helicase activity
Process	GO:0032508	DNA duplex unwinding

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	expressed	0.47	0.78
Trophozoite	expressed	0.52	0.71
Schizont	expressed	0.26	0.84
Gametocyte	expressed	0.76	0.60

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0807100

Resistome Missense Mutations: N536K

Resistome Compounds with Missense Mutations: AN13762

Resistome # Samples with Disruptive Mutations: 1 (1 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.964 | MFS: -2.327 | #Insertions: 1

PlasmoGEM Phenotype: Essential (Pb ortholog: PBANKA_1223500)

Relative Growth Rate: 0.08 ± 0.09
Confidence: 6.22

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IAR1

"Best" AlphaFill ligand hit: ANP (phosphoaminophosphonic acid-adenylate ester, Local RMSD=0.03) with 6B4J (Global RMSD=7.70)

BRENDA EC Inhibitors:

EC #	Name	EC Inhibitors
3.6.4.12	DNA helicase	No BRENDA inhibitors
3.6.4.13	RNA helicase	ATPADPUDPAMPGTPGDPUTPKClNEMCDPITPTDP...

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_134689

No human ortholog(s)

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	13	71	27	62
disruptive	52	152	98	188
missense	34	128	90	161

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	28	114	46	78
disruptive	91	322	140	317
missense	59	256	115	215

PlasmoDB Total SNPs: 251

Non-coding: 78 | Synonymous: 111 | Nonsynonymous: 62 | Stop Codon: 0

Protein Information

Protein Length: 1321 | Molecular Weight (kDa): 157.197

UniProt ID(s): A0A2D1GTQ5, A0A2D1GTQ8, Q8IAR1

PDB ID(s): None

Isoelectric Point: 9.46

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR001650	Helicase, C-terminal
InterPro	IPR011545	DEAD/DEAH box helicase domain
InterPro	IPR014001	Helicase superfamily 1/2, ATP-binding domain
InterPro	IPR027417	P-loop containing nucleoside triphosphate hydrolase
PFam	PF00270	DEAD/DEAH box helicase domain
PFam	PF00271	Helicase, C-terminal
Superfamily	SSF52540	P-loop containing nucleoside triphosphate hydrolase