Product Description: serine protease DegP
SignalP Peptide: MDIIFCTPTYCKIMLMIIMLISLRTRCDT
# Transmembrane Domains: 0
EC Numbers: 3.4.21.- (Serine endopeptidases.)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0005829 | cytosol |
| Component | GO:0044164 | host cell cytosol |
| Component | GO:0020002 | host cell plasma membrane |
| Component | GO:0005739 | mitochondrion |
| Component | GO:0020003 | symbiont-containing vacuole |
| Function | GO:0005515 | protein binding |
| Function | GO:0004252 | serine-type endopeptidase activity |
| Process | GO:0006508 | proteolysis |
| Process | GO:0006979 | response to oxidative stress |
| Process | GO:0009266 | response to temperature stimulus |
| Process | GO:0009410 | response to xenobiotic stimulus |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | possibly expressed | 0.16 | 0.92 |
| Trophozoite | expressed | 0.23 | 0.87 |
| Schizont | expressed | 1.11 | 0.48 |
| Gametocyte | expressed | 0.20 | 0.89 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0807700
Resistome Missense Mutations: None
Resistome Compounds with Missense Mutations: None
Resistome # Samples with Disruptive Mutations: 1 (0 missense, 0 "interesting" missense)
Zhang Phenotype: Mutable in CDS
MIS: 1 | MFS: 0.991 | #Insertions: 7
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IAR5
"Best" AlphaFill ligand hit: CXS (3-cyclohexyl-1-propylsulfonic acid, Local RMSD=0.30) with 5JD8 (Global RMSD=4.71)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 3.4.21.- | Ex: physarolisin | SDSCysDFPGAWSTIDANE64KD1EDTAPMSFDMSOTLCK... |
No evidence of orthology to BindingDB entries
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 16 | 30 | 24 | 55 |
| disruptive | 36 | 76 | 49 | 120 |
| missense | 25 | 60 | 39 | 85 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 23 | 61 | 50 | 51 |
| disruptive | 61 | 188 | 87 | 235 |
| missense | 33 | 130 | 65 | 124 |
PlasmoDB Total SNPs: 284
Non-coding: 152 | Synonymous: 82 | Nonsynonymous: 50 | Stop Codon: 0
Protein Length: 870 | Molecular Weight (kDa): 101.494
PDB ID(s): None
Isoelectric Point: 9.84
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR001940 | Peptidase S1C |
| InterPro | IPR009003 | Peptidase S1, PA clan |
| InterPro | IPR041517 | Protease Do-like, PDZ domain |
| PFam | PF13365 | Protease Do-like, PDZ domain |
| PFam | PF17815 | |
| Superfamily | SSF50494 | Peptidase S1, PA clan |
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 24494818 | A secretory multifunctional serine protease, DegP of Plasmodium falciparum, playsan important role in thermo-oxidative stress, parasite growth and development. | Sharma S, Jadli M, ..., Malhotra P | 10.1111/febs.12732 |
| 32968076 | Genetic screens reveal a central role for heme metabolism in artemisininsusceptibility. | Harding CR, Sidik SM, ..., Lourido S | 10.1038/s41467-020-18624-0 |
| 34494883 | A Prioritized and Validated Resource of Mitochondrial Proteins in PlasmodiumIdentifies Unique Biology. | van Esveld SL, Meerstein-Kessel L, ..., Huynen MA | 10.1128/mSphere.00614-21 |