PF3D7_0807800 (RPN10)

Genome location: Pf3D7_08_v3:399,105..401,398(-)

Genome classification: Core

Function and Localization

Product Description: 26S proteasome regulatory subunit RPN10, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0005829	cytosol
Component	GO:0005634	nucleus
Component	GO:0008540	proteasome regulatory particle, base subcomplex
Function	GO:0031593	polyubiquitin modification-dependent protein binding
Process	GO:0043248	proteasome assembly
Process	GO:0043161	proteasome-mediated ubiquitin-dependent protein catabolic process

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	expressed	N/A	N/A
Ring	expressed	0.37	0.82
Trophozoite	expressed	0.66	0.63
Schizont	expressed	0.74	0.58
Gametocyte	expressed	0.62	0.64

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0807800

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Non - Mutable in CDS

MIS: 0.144 | MFS: -2.93 | #Insertions: 0

PlasmoGEM Phenotype: N/A

RMgmDB ABS Phenotype: N/A

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: Q8IAR6

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102002

Most Similar Human Ortholog: A6PVX3

TM-align score: 0.86 | RMSD: 1.96

Seq Identity: 0.33 | Length: 188 / 481

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000159352	proteasome 26S subunit, non-ATPase 4

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	4	19	14	41
disruptive	15	13	15	51
missense	9	6	12	46

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	8	42	24	44
disruptive	23	55	43	110
missense	12	38	35	74

PlasmoDB Total SNPs: 182

Non-coding: 129 | Synonymous: 44 | Nonsynonymous: 9 | Stop Codon: 0

Protein Information

Protein Length: 481 | Molecular Weight (kDa): 55.067

UniProt ID(s): Q2KNU4, Q2KNU5, Q8IAR6

PDB ID(s): None

Isoelectric Point: 4.45

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR002035	von Willebrand factor, type A
InterPro	IPR014624	Predicted 26S proteasome regulatory complex, non-ATPase subcomplex, subunit s5a, Plasmodium
InterPro	IPR027040	Proteasome subunit Rpn10
InterPro	IPR036465	von Willebrand factor A-like domain superfamily
PFam	PF13519	von Willebrand factor, type A
Superfamily	SSF53300	von Willebrand factor A-like domain superfamily

PMID	Title	Authors	DOI/Link
16267556	A protein interaction network of the malaria parasite Plasmodium falciparum.	LaCount DJ, Vignali M, ..., Hughes RE	10.1038/nature04104
23181666	Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum.	Oehring SC, Woodcroft BJ, ..., Voss TS	10.1186/gb-2012-13-11-r108