Product Description: tyrosine--tRNA ligase
SignalP Peptide: N/A
# Transmembrane Domains: 0
EC Numbers: 6.1.1.1 (Tyrosine--tRNA ligase)
Curated GO (PlasmoDB):
| Type | GO Term | Name |
|---|---|---|
| Component | GO:0044164 | host cell cytosol |
| Component | GO:0005634 | nucleus |
| Function | GO:0046789 | host cell surface receptor binding |
| Function | GO:0004831 | tyrosine-tRNA ligase activity |
| Process | GO:0006437 | tyrosyl-tRNA aminoacylation |
Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):
| Stage | LR class | MCA mean | MCA prop. zeros |
|---|---|---|---|
| Sporozoite | expressed | N/A | N/A |
| Ring | expressed | 0.30 | 0.85 |
| Trophozoite | expressed | 0.87 | 0.53 |
| Schizont | expressed | 0.50 | 0.68 |
| Gametocyte | expressed | 0.40 | 0.77 |
More info:
Old (Pf3D7v3) Gene ID: PF3D7_0807900
Resistome Missense Mutations: S234C
Resistome Compounds with Missense Mutations: ML901
Resistome # Samples with Disruptive Mutations: 3 (3 missense, 3 "interesting" missense)
Zhang Phenotype: Non - Mutable in CDS
MIS: 0.279 | MFS: -2.675 | #Insertions: 0
PlasmoGEM Phenotype: N/A
RMgmDB ABS Phenotype: N/A
More info: PhenoPlasm Link
AlphaFill Uniprot ID: Q8IAR7
"Best" AlphaFill ligand hit: ANL (aniline, Local RMSD=0.34) with 2OV4 (Global RMSD=4.91)
BRENDA EC Inhibitors:
| EC # | Name | EC Inhibitors |
|---|---|---|
| 6.1.1.1 | tyrosine-tRNA ligase | AMPPCMBsulfatefisetintRNATyrchrysinchlorideacacetintyrosinoldiphosphateresveratrolkaempferide... |
Orthology to BindingDB Entries:
| UniProt | Protein Name | Species | Homology Source | BindingDB Ligands |
|---|---|---|---|---|
| P54577 | Tyrosine--tRNA ligase, cytoplasmic | Homo sapiens | OMA, OrthoDB | CHEMBL98622CHEMBL95616CHEMBL99347CHEMBL318019... |
Ortholog Group (OrthoMCL): OG6_101224
Most Similar Human Ortholog: P54577
TM-align score: 0.86 | RMSD: 2.04
Seq Identity: 0.30 | Length: 341 / 373
All Human Orthologs (OrthoMCL):
| Gene ID | Description |
|---|---|
| ENSG00000134684 | tyrosyl-tRNA synthetase 1 |
Protein Length: 373 | Molecular Weight (kDa): 43.453
UniProt ID(s): Q8IAR7
PDB ID(s): 3VGJ, 7ROR, 7ROS, 7ROT
Isoelectric Point: 7.5
Protein Domain Annotations:
| Source | Family ID | Description |
|---|---|---|
| InterPro | IPR002305 | Aminoacyl-tRNA synthetase, class Ic |
| InterPro | IPR023617 | Tyrosine-tRNA ligase, archaeal/eukaryotic-type |
| PFam | PF00579 | Aminoacyl-tRNA synthetase, class Ic |
| Superfamily | SSF52374 | N/A |
MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:
Homozygous genotype calls only
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 18 | 7 | 12 |
| disruptive | 4 | 17 | 8 | 22 |
| missense | 4 | 17 | 8 | 20 |
Any inclusion in genotype call
| variant type | common | rare | doubleton | singleton |
|---|---|---|---|---|
| synonymous | 3 | 36 | 12 | 18 |
| disruptive | 6 | 45 | 30 | 87 |
| missense | 4 | 35 | 25 | 57 |
PlasmoDB Total SNPs: 119
Non-coding: 104 | Synonymous: 10 | Nonsynonymous: 5 | Stop Codon: 0
| PMID | Title | Authors | DOI/Link |
|---|---|---|---|
| 16267556 | A protein interaction network of the malaria parasite Plasmodium falciparum. | LaCount DJ, Vignali M, ..., Hughes RE | 10.1038/nature04104 |
| 22068597 | Malaria parasite tyrosyl-tRNA synthetase secretion triggers pro-inflammatoryresponses. | Bhatt TK, Khan S, ..., Sharma A | 10.1038/ncomms1522 |
| 23181666 | Organellar proteomics reveals hundreds of novel nuclear proteins in the malariaparasite Plasmodium falciparum. | Oehring SC, Woodcroft BJ, ..., Voss TS | 10.1186/gb-2012-13-11-r108 |
| 30379851 | Identification of Plasmodium falciparum nuclear proteins by mass spectrometry andproposed protein annotation. | Briquet S, Ourimi A, ..., Vaquero C | 10.1371/journal.pone.0205596 |