PF3D7_0808400 (SEC28)

Genome location: Pf3D7_08_v3:422,708..424,763(+)

Genome classification: Core

Function and Localization

Product Description: coatomer subunit epsilon, putative

SignalP Peptide: N/A

# Transmembrane Domains: 0

EC Numbers: None

Curated GO (PlasmoDB):

Type	GO Term	Name
Component	GO:0030126	COPI vesicle coat
Process	GO:0006888	endoplasmic reticulum to Golgi vesicle-mediated transport
Process	GO:0006891	intra-Golgi vesicle-mediated transport

Expression by stage (LR - Le Roch et al., and MCA - Malaria Cell Atlas):

Stage	LR class	MCA mean	MCA prop. zeros
Sporozoite	not expressed	N/A	N/A
Ring	not expressed	0.29	0.86
Trophozoite	possibly expressed	0.42	0.74
Schizont	possibly expressed	0.43	0.73
Gametocyte	not expressed	0.28	0.83

More info:

Resistome Mutations

Old (Pf3D7v3) Gene ID: PF3D7_0808400

Resistome Missense Mutations: None

Resistome Compounds with Missense Mutations: None

Resistome # Samples with Disruptive Mutations: 0 (0 missense, 0 "interesting" missense)

Essentiality (ABS)

Zhang Phenotype: Mutable in CDS

MIS: 0.92 | MFS: -2.792 | #Insertions: 2

PlasmoGEM Phenotype: Dispensable (Pb ortholog: PBANKA_1222300)

Relative Growth Rate: 0.90 ± 0.14
Confidence: 5.26

RMgmDB ABS Phenotype: Different from wild type (Pb ortholog: PBANKA_1222300)

Modification: Disrupted | RMgm-3284

More info: PhenoPlasm Link

Binding Evidence

AlphaFill Uniprot ID: C0H4T1

"Best" AlphaFill ligand hit: No AlphaFill hits

No associated EC numbers

No evidence of orthology to BindingDB entries

Orthology Information

Ortholog Group (OrthoMCL): OG6_102500

Most Similar Human Ortholog: O14579

TM-align score: 0.89 | RMSD: 2.12

Seq Identity: 0.19 | Length: 273 / 278

All Human Orthologs (OrthoMCL):

Gene ID	Description
ENSG00000105669	COPI coat complex subunit epsilon

Genetic Variation

MalariaGEN Pf7 (worldwide samples) # unique SNV/indels:

Homozygous genotype calls only

variant type	common	rare	doubleton	singleton
synonymous	2	5	2	10
disruptive	5	21	9	25
missense	5	20	9	24

Any inclusion in genotype call

variant type	common	rare	doubleton	singleton
synonymous	3	16	7	9
disruptive	9	45	26	68
missense	9	40	21	41

PlasmoDB Total SNPs: 173

Non-coding: 155 | Synonymous: 13 | Nonsynonymous: 5 | Stop Codon: 0

Protein Information

Protein Length: 278 | Molecular Weight (kDa): 33.093

UniProt ID(s): C0H4T1, Q2KNS1

PDB ID(s): None

Isoelectric Point: 4.33

Protein Domain Annotations:

Source	Family ID	Description
InterPro	IPR006822	Coatomer, epsilon subunit
PFam	PF04733	N/A
Superfamily	N/A	N/A

PMID	Title	Authors	DOI/Link
10744762	Impaired proteasome function rescues thermosensitivity of yeast cells lacking thecoatomer subunit epsilon-COP	Kimata Y, Higashio H, Kohno K	10.1074/jbc.275.14.10655
17101773	Involvement of specific COPI subunits in protein sorting from the late endosometo the vacuole in yeast	Gabriely G, Kama R, Gerst JE	10.1128/MCB.00577-06